低溫處理及液體培養對水稻花葯癒傷組織形成之效果
Other Title
The Effects of Cold Shock and Liquid Medium on Callus Formation in Rice Anther Culture
Journal
中華農業研究
Journal Volume
33
Journal Issue
1
Pages
24-29
Date Issued
1984-03-03
Date
1984-03-03
Author(s)
蔡新聲
陳良築
H.S. Tsay
L.J. Chen
Abstract
水稻花葯培養前，將附有劍葉之稻穗經 10℃, 7 天之低溫處理，可提高花葯callus形成率一倍。花葯接種於培養基後再行低溫處理則無效。低溫處理花葯所得callus，分化之綠苗，有較多單倍體而較少雙倍體的趨勢。
液體培養不但無益於花葯 callus 之誘導，且形成之 callus 分化力極差，故不適宜水稻花葯培養。
Rice culm with flag leaf was pretreated by low temperature at 10℃ for 7 days. Anthers taken from these pretreated flowers were cultured on solid medium. It was found that cold-shocked anthers produced more callus (2-fold) than those without pretreatment. The effect was not significant when pretreatment was done after anthers have been cultured on medium. Callus initiated from cold-shocked anthers produced more haploid plants and less diploid plants than the callus initiated from anthers without pretreatment.
Due to the low differentiation ability of callus initiated from anthers cultured in the liquid medium, it is considered that liquid suspension culture is not suitable for rice anther culture.
Publisher
農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
journal_arc_33-1-3.pdf
Size
682.6 KB
Format
Adobe PDF
Checksum
(MD5):60e1622354511440ca866794e80b831c