|Title:||低溫處理及液體培養對水稻花葯癒傷組織形成之效果||Other Titles:||The Effects of Cold Shock and Liquid Medium on Callus Formation in Rice Anther Culture||Authors:||蔡新聲
|Issue Date:||3-Mar-1984||Publisher:||農業試驗所||Journal Volume:||33||Journal Issue:||1||Start page/Pages:||24-29||Source:||中華農業研究||Abstract:||
水稻花葯培養前，將附有劍葉之稻穗經 10℃, 7 天之低溫處理，可提高花葯callus形成率一倍。花葯接種於培養基後再行低溫處理則無效。低溫處理花葯所得callus，分化之綠苗，有較多單倍體而較少雙倍體的趨勢。
液體培養不但無益於花葯 callus 之誘導，且形成之 callus 分化力極差，故不適宜水稻花葯培養。
Rice culm with flag leaf was pretreated by low temperature at 10℃ for 7 days. Anthers taken from these pretreated flowers were cultured on solid medium. It was found that cold-shocked anthers produced more callus (2-fold) than those without pretreatment. The effect was not significant when pretreatment was done after anthers have been cultured on medium. Callus initiated from cold-shocked anthers produced more haploid plants and less diploid plants than the callus initiated from anthers without pretreatment.
Due to the low differentiation ability of callus initiated from anthers cultured in the liquid medium, it is considered that liquid suspension culture is not suitable for rice anther culture.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
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