應用核酸探針系統檢測瓜類褪綠黃化病毒
Other Title
A DNA Probing System for the Detection of Cucurbit Chlorotic Yellows Virus
Resource
台灣農業研究 63(4): 259-266
Journal of Taiwan Agricultural Research 63(4): 259-266
Journal
台灣農業研究
Journal Volume
63
Journal Issue
4
Pages
259-266
Date Issued
2014-12-01
Date
2014-12-01
Author(s)
張文學
鄧汀欽
Wen-Shiue Chang
Ting-Ching Deng
Abstract
本研究以微珠核酸探針系統應用於瓜類褪綠黃化病毒(CCYV)之檢測，並進行田間洋香瓜是否感染CCYV之測試。此微珠核酸探針系統之檢測包含可擴增CCYV病毒鞘蛋白(CP)基因及一組植物葉綠體來源基因(ndhB)，分別設計一組引子對，進行反轉錄聚合酵素連鎖反應及微珠核酸探針系統反應。
In this paper, a PCR assay coupled with microspheres to rapidly screen field samples for ＂Cucurbit chlorotic yellows virus＂ (CCYV) survey on melons was developed. The RT-PCR was designed to have the same binding sites for the forward and reverse primers of the CCYV viral coat protein (CP) gene as the target amplicon, but it had a unique internal sequence used for the probe site.
Subjects
瓜類褪綠黃化病毒 (CCYV)
核酸探針
檢測
Cucurbit chlorotic yellows virus (CCYV)
Nucleic acid probe
Detection
Publisher
農業試驗所
Type
journal article
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