台灣山藥上分離的蠶豆萎凋病毒兩個株系之比較鑑定
Other Title
Comparative Characterization of Two Isolates of Broad bean wilt virus 2 Infecting Yams (Dioscorea spp.) in Taiwan
Journal
台灣農業研究
Journal Volume
63
Journal Issue
4
Pages
282-290
Date Issued
2014-12-04
Date
2014-12-04
Author(s)
鄧汀欽
林羿廷
蔡錦慧
廖吉彥
高瑞隆
Ting-Chin Deng
Yi-Ting Lin
Chin-Hui Tsai
Jye-Yann Liao
Ruei-Lung Kao
Abstract
採集畸形嵌紋的「台農1號」山藥病株及黃化嵌紋病徵的日本山藥病株，接種奎藜經單斑分離後分別得到病毒分離株“YA3＂與“Yam-7＂。陰染法電子顯微鏡觀察，均為球形病毒。兩者都可被蠶豆萎凋病毒2(Broadbean wilt virus 2; BBWV-2)抗體檢出。
Leaf samples with mosaic and malformation on ＂Dioscorea alata＂ and that with yellowing and mosaic symptoms on ＂Diosocoreab atatas＂ were collected for inoculation tests. Both of them developed local lesions on ＂Chenopodium quinoa＂, and two virus isolates 'YA3' and 'Yam-7' were obtained through single lesion isolation.
Subjects
豆類病毒屬
引子對
反轉錄聚合酶鏈式反應
核苷酸序列
親緣分析
Fabavirus
Primer
RT-PCR
Nucleotide sequence
Phylogenetic analysis
Publisher
農業試驗所
Type
journal article
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