培植體、植物生長調節劑及光照處理對丹參不定芽誘導之影響

Abstract

本研究利用丹參 (Salvia miltiorrhiza) 組織培養苗之葉柄和葉片培植體進行不定芽的誘導試驗。葉柄培植體於含有1 mg L-1 芐腺嘌呤 (N6-benzyladenine; BA) 與0.5 mg L-1 奈乙酸 (α-naphthaleneacetic acid; NAA) 之MS (Murashige & Skoog 1962) 培養基中培養6 wk 後不定芽形成率達100%，平均每個培植體可形成3.7 個不定芽。 Petiole and leaf explants derived from in vitro Salvia miltiorrhiza were used for shoot regeneration in this study. The highest adventitious shoot formation rate of 100% with 3.7 shoots/explant in average was obtained from petiole segments cultured on Murashige and Skoog’s (MS) medium containing 1 mg L-1 N6-benzyladenine (BA) and 0.5 mg L-1 α-naphthaleneacetic acid (NAA) for 6 wk of culture.