培植體、植物生長調節劑及光照處理對丹參不定芽誘導之影響
Other Title
Influence of Explant, Plant Growth Regulator and Illumination on Adventitious Shoot Induction of In Vitro Cultured Salvia miltiorrhiza
Journal
台灣農業研究
Journal Volume
65
Journal Issue
4
Pages
384-394
Date Issued
2016-12-04
Date
2016-12-04
Author(s)
陳威臣
曹進義
夏奇鈮
Uei-Chern Chen
Jhin-Yi Tsao
Chi-Ni Hsia
Abstract
本研究利用丹參 (Salvia miltiorrhiza) 組織培養苗之葉柄和葉片培植體進行不定芽的誘導試驗。葉柄培植體於含有1 mg L-1 芐腺嘌呤 (N6-benzyladenine; BA) 與0.5 mg L-1 奈乙酸 (α-naphthaleneacetic acid; NAA) 之MS (Murashige & Skoog 1962) 培養基中培養6 wk 後不定芽形成率達100%，平均每個培植體可形成3.7 個不定芽。
Petiole and leaf explants derived from in vitro Salvia miltiorrhiza were used for shoot regeneration in this study. The highest adventitious shoot formation rate of 100% with 3.7 shoots/explant in average was obtained from petiole segments cultured on Murashige and Skoog’s (MS) medium containing 1 mg L-1 N6-benzyladenine (BA) and 0.5 mg L-1 α-naphthaleneacetic acid (NAA) for 6 wk of culture.
Subjects
直接器官形成
間接器官形成
癒合組織
大量繁殖
Directly organogenesis
Indirectly organogenesis
Callus formation
Micropropagation
Publisher
農業試驗所
Type
journal article
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