建立馬鈴薯多重聚合酶連鎖反應檢測技術

Other Title
Establishment and Application of Multiplex Polymerase Chain Reaction for Simultaneous Detection of Transgenic Potatoes
Resource
台灣農業研究68(1):16-27
Journal of Taiwan Agricultural Research 68(1):16-27
Journal
台灣農業研究
Journal Volume
68
Journal Issue
1
Pages
16-27
Date Issued
2019-03-02
Date
2019-03-02
Author(s)
馮昱維
陳烈夫 
林彥君
Yu-Wei Feng
Lit-Fu Chan 
Shu Chen 
Yen-Chun Lin
DOI
URI
Abstract
馬鈴薯（Solanum tuberosum L.）為我國常見的食用及加工作物，其塊莖在工業和飼料上具有很高的應用價值。本試驗以馬鈴薯物種專一性（taxon-specific）內生性基因尿苷二磷酸葡萄糖焦磷酸化酶（UDP-glucose pyrophosphorylase; UGPase）序列與基因轉殖馬鈴薯品項之專一性序列（event-specific sequence）建立多重聚合酶連鎖反應（multiplex polymerase chain reaction; mPCR）檢測技術。 Potato (Solanum tuberosum L.) is known as one of the most important food crops in the world and has high application in industry and feed. In this study, we present a multiplex polymerase chain reaction (mPCR) method for simultaneous detection of genetically modified (GM) potatoes. Quadruplex PCR assay targeting three event-specific sequences and the taxon-specific endogenous reference gene (UDP-glucose pyrophosphorylase; UGPase) were established.
Subjects
馬鈴薯
多重聚合酶連鎖反應
檢驗方法
Potato
mPCR
Detecting method
Publisher
農業試驗所
Type
journal article
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