Repeated Dose 90-Day Feeding Study of Whole Fruits of Genetically Modified Papaya Resistant to Papaya Ringspot Virus in Rats
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
63
Journal Issue
4
Pages
1286-1292
Date Issued
2015-02
Date
2015-02
Author(s)
Hsin-Tang Lin
Gow-Chin Yen
Wei-Cheng Lee
Yi-Ting Tsai
Jhaol-Huei Wu
Shyi-Dong Yeh
Ying-Huey Cheng
Shih-Chieh Chang
Jiunn-Wang Liao
Abstract
Genetically modified (GM) papaya plants resistant to infection by Papaya ringspot virus (PRSV) have been successfully generated by cloning the coat protein (CP) gene of PRSV to increase fruit production. In this study, the GM papaya line 823-2210 was used to conduct a 90-day feeding toxicity study and compared to its parent plant of non-GM papaya, Tainung-2 (TN-2) based on the experimental guidance reported by the European Food Safety Authority.1 Ten male and 10 female Sprague-Dawley albino rats were gavaged at low (1 g/kg bw) and high (2 g/kg bw) doses of non-GM and GM lyophilized papaya fruits for 90 days. Hematology, coagulation, biochemistry, urinalysis, and pathology were examined in all animals. Although some differences were found in feed consumption, hematology, and serum chemistry examinations between non-GM and GM papaya, the results were within historical control values and not considered biologically significant in rats. In addition, there were no treatment-related gross or microscopic lesions in male or female rats attributable to the non-GM or GM papaya fruit. This 90-day feeding study of GM papaya fruit did not reveal adverse effects in rats and indicates that GM papaya fruits may be substantially equivalent to their non-GM parent plants.
Subjects
genetically modified
papaya
PRSV
subchronic feeding toxicity
rats
Publisher
Easton, Pa., American Chemical Society, Books and Journals Division
Type
journal article