Application of internal transcribed spacers and maturase K markers for identifying Anoectochilus, Ludisia, and Ludochilus

J.-R. Chen; Y.-J. Shiau

doi:10.1007/s10535-015-0520-3

Application of internal transcribed spacers and maturase K markers for identifying Anoectochilus, Ludisia, and Ludochilus

Journal

Biologia Plantarum

Journal Volume

59

Journal Issue

3

Pages

485-490

Date Issued

2015-09

Date

2015-09

Author(s)

J.-R. Chen

Y.-J. Shiau

DOI

10.1007/s10535-015-0520-3

URI

https://scholars.tari.gov.tw/handle/123456789/1430

http://bp.ueb.cas.cz/artkey/bpl-201503-0010_application-of-internal-transcribed-spacers-and-maturase-k-markers-for-identifying-anoectochilus-l.php

Abstract

Internal transcribed spacer (ITS) regions and maturase K (matK) sequence polymorphisms provide an efficient tool for discrimination and conservation of genetic resources of Anoectochilus species. The objectives of this study were to develop markers specifically distinguishing A. formosanus Hayata from closely related A. koshunensis Hayata, A. roxburghii (Wall.) Lindl., and Ludisia discolor (Ker Gawl.) A. Rich. and to identify a molecular phylogenetic relationship of a new intergeneric BC1F1 hybrid - Ludochilus Jin-Chai. Specific primers for nuclear ITS regions and chloroplast matK sequences were designed and converted into cleaved amplified polymorphic sequence (CAPS) markers. Results show that the matK sequences obtained corresponded to pseudogenes and that their digestion with enzyme HinfI revealed a polymorphic pattern in A. formosanus and A. koshunensis. The pedigree of Lud. Jin-Chai, which was derived from the cross between Lus. discolor and A. formosanus, was also confirmed based on ITS and matK CAPS markers.

Subjects

chloroplast matK sequence

intergeneric hybrid

jewel orchids

plant phylogenetic analysis

Publisher

Acad Sciences Czech Republic

Type

journal article