Effects of Antrodia camphorata on Alcohol Clearance and Antifibrosis in Livers of Rats Continuously Fed Alcohol

Journal
Journal of Agricultural and Food Chemistry
Journal Volume
59
Journal Issue
8
Pages
4248-4254
Date Issued
2011-04
Date
2011-04
Author(s)
Min-Tze Wu
Bor-Show Tzang
Yuan-Yen Chang
Chih-Hsien Chiu
Wen-Yu Kang
Chia-Hsin Huang
Yi-Chen Chen
DOI
URI
Abstract
Alcoholic fatty liver disease (AFLD) is the result of an excessive or chronic consumption of alcohol. Nine male Wistar rats per group were randomly assigned to one of the following drinking treatments: a 20% (w/w) alcohol solution (ALC); a 20% (w/w) alcohol solution cotreated with 0.25 g silymarin/kg BW/day; or a 20% (w/w) alcohol solution cotreated with 0.025 g Niuchangchih (Antrodia camphorata)/kg BW/day for 4 weeks. Rats with cotreatments of silymarin or Niuchangchih had smaller (p < 0.05) relative liver size, less (p < 0.05) liver lipid accumulation, and lower (p < 0.05) liver damage indices [aspartate aminotransferase (AST) and alkaline phosphatase (ALP) values]. In the regulation of alcohol metabolism, the lower serum alcohol level was observed only in alcohol-fed rats supplemented with Niuchangchih. Meanwhile, cotreatment of silymarin or Niuchangchih increased (p < 0.05) CAT and ALDH activities but did not (p > 0.05) affect ADH and CYP2E1 expressions, which accelerate alcohol metabolism in the body. Additionally, neither silymarin nor Niuchangchih (p > 0.05) influenced serum/hepatic MMP-2 activities and NF-κB, AP1, and α-SMA gene expressions, but serum/hepatic MMP-9 activities and TNF-α, KLF-6, and TGF-β1 gene expressions of alcohol-fed rats were down-regulated (p < 0.05) by silymarin or Niuchangchih, which also could explain the lower liver damage observed in rats chronically fed alcohol.
Subjects
alcoholic fatty liver
Antrodia camphorata
liver lipid accumulation
liver damage index
alcohol metabolism
fibrosis
Publisher
American Chemical Society
Type
journal article