藥用植物苦參微體繁殖及瓶苗馴化研究

林資哲; 夏奇鈮; 陳威臣; 葉茂生; Tzu-Che Lin; Chi-Ni Hsia; Uei-Chern Chen; Mau-Shing Yeh; 林資哲;夏奇鈮;陳威臣;葉茂生; Tzu-Che Lin;Chi-Ni Hsia;Uei-Chern Chen;Mau-Shing Yeh

藥用植物苦參微體繁殖及瓶苗馴化研究

Other Title

Micropropagation and Acclimation Studies on Medicinal Plant of Sophora flavescens Ait.

Journal

作物、環境與生物資訊

Journal Volume

7

Journal Issue

3

Pages

145-152

Date Issued

2010-09

Date

2010-09

Author(s)

林資哲

夏奇鈮

陳威臣

葉茂生

Tzu-Che Lin

Chi-Ni Hsia

Uei-Chern Chen

Mau-Shing Yeh

URI

https://scholars.tari.gov.tw/handle/123456789/14551

Abstract

藥用植物苦參(Sophora flavescens Ait.)大多生長於野外，天然結實之種子發芽不易，臺灣目前亦少有商業形式的栽培，市場所需藥材皆仰賴中國大陸進口。臺灣如欲大量栽種苦參，必須克服種子發芽率低之問題外，亦可利用組織培養技術建立苦參大量繁殖方法，以提供商業栽培所需之種苗。本研究以花蓮縣生產之苦参種子建立無菌瓶苗，再以瓶苗進行芽體增殖、瓶苗發根及瓶苗出瓶馴化等試驗。以兩種方法比較促進苦參種子發芽效果，一以1% HCl 溶液浸泡種子30 min，另一以 60℃溫水浸泡種子60 min。在無菌播種後的結果顯示，以60℃溫水處理之苦參種子，接種在1/2 MS 鹽類配方培養基中，發芽率最高可達73.33%。瓶苗繁殖方面，以莖節之瓶苗莖段為培植體，接種於添加0-2.0 mg L-1 BA (benzyladenine) 或 TDZ (thidiazuron)之 MS 基礎鹽類培養基中培養，結果以 2.0 mg L-1 BA 處理組之芽體誘導率最高(96.67%)，平均每個莖段可增殖10.4 個芽體。瓶苗發根方面，待芽體生長至1.5-2 cm 後，切取單一芽體接種在添加0-1.0 mg L-1 NAA (α-naphthalenacetic acid) 或IBA (indole-3-butyric acid)之 1/2 MS 培養基中誘導發根，結果以1.0 mg L-1 NAA 處理之瓶苗具最高之根數及根長表現。將已發根高約4-5 cm 之組培苗移出瓶外種植，調查瓶苗期間施以不同濃度NAA 與IBA 之發根植株，經過2 及4 wks 馴化處理後的生長情形，結果顯示，瓶內發根次佳之處理 (0.5 mg L-1 NAA) 在經4 wks 馴化後其小苗生長較瓶內發根最佳之1 mg L-1 NAA 處理表現更佳。顯見在瓶內發根階段，雖然較高濃度的auxin 處理下有利於根的生長，但過高之生長素濃度可能不利於後續植株之生長。因此，建議在瓶內發根培養時使用較低之auxin 濃度即可，對於出瓶後小苗之生長可能更為有利。 Sophora flavescens Ait. is a commonly used medicinal plant in Taiwan. However, roots of S. flavescens for medicinal needs are mostly imported from China. Therefore, studies on how to overcome the problem of low seed germination and provide sufficient seedings of S. flavescens for commercial cultivation were carried out. Seeds of S. flavescens were collected from Hualien Hsien of Taiwan and were used to establish aseptic explants, shoot induction, proliferation, rooting and ex vitro acclimation. Two methods were examined for the function in increasing seed germination. In the first one, seeds were soaking in 1 % HCl solution for 30 min, and the second one used 60℃ warm water for 60 min. The germination rate went up to 73.33% on a 1/2 MSbased salt medium in the second method. Nodal explants were cultured in a MS-based salt medium containing 0-2.0 mg L-1 BA (benzyladenine) or TDZ (thidiazuron) for shoot proliferation. The highest induction rate (96.67%) was obtained at 2.0 mg L-1 BA with 10.4 shoots explants-1. Shoots with 1.5-2.0 cm in length were subcultured into a 1/2 MS-based salt medium containing 0-1.0 mg L-1 NAA (α-naphthalenacetic acid) or IBA (indole-3-butyric acid) for rooting. Both the highest root number and root length were obtained at 1.0 mg L-1 NAA treatment. Seeding growth after 2 to 4-weeks ex vitro acclimation was recorded. After 4-week acclimation, the second best rooting treatment in vitro (0.5 mg L-1 NAA) was found superior than 1 mg L-1 NAA treatment. Although higher auxin treatment might be benifical to in vitro rooting, a lower concentration of auxin for ex vitro seeding growth was recommended.

Subjects

苦參

藥用植物

微體繁殖

馴化

Sophora flavescens Ait.

Medicinal plant

Micropropagation

Acclimation

Publisher

台灣農藝學會

Type

journal article

File(s)

Name

7-3-145-152.pdf

Size

1.72 MB

Format

Adobe PDF

Checksum

(MD5):7ac21c4176d35a29c9488ab576174e38