綬草之種苗量化

Abstract

綬草為具觀賞與藥用的蘭科植物，由於人為採集目前已不常見，為了大量繁殖綬草的種苗，進行組織培養及菌根菌輔助綬草繁殖技術研發，結果顯示以授粉後20天成熟度果莢播種發芽率較佳，以l/2或l／榭S培養基添加MES及pePtone可提高種子發芽率。實生苗培植體在特定基鹽與蔗糖濃度的培養下，存活率可高達70.8％且生育良好，無褐化及玻璃質化現象發生。細胞分裂素與椰子水處理均可誘導培植體產生芽體，最高可獲得7個不定芽形成，過高濃度的細胞分裂素處理，培植體葉月易發生肥大扭曲且出現白化現象。組培苗經不同馴化處理方式其存活率可達85％以上。在菌根菌輔助綬草繁殖技術方面，以菌根菌先接種於培養基上，7天後播種其上綬草種子之發芽率73.2％最高，播種密度以0.2 g•m-2的種子用量表現最佳，栽培介質以溪砂加牛糞可使栽培之綬草收成達最高之鮮重。上述建立之技術，以非專屬授權方式技轉業者使用，生產綬草種苗。 Spiranthes sinensis is an orchid with ornamental and medicinal functions. Because people collected a lot, it has rarely seen. We established the tissue culture propagation system and used mycorrhizal fungi to increase seed germination to mass production seedlings of Spiranthes sinensis. The results showed that 20 days after pollination, the seed germination rate was higher than other treatments. Using 1/2 or 1/4MS with MES and peptone medium could increase seed germination. The explants of Spiranthes sinensis were grown well in fit salt-base strength and sucrose concentration, the survival rate of explants could be as high as 70.8%, and there were no browning and vitrification. Cytokinin and coconut milk could induce shoot formation. Using fit plant growth regulator combined treatment could induce 7.0 shoots formation and there were shoot buds formed in the base of explants. With high cytokine in concentration treatment, the leaves of explants would become curved or whiteness. The survival rate of plantlets could up to 85% in all acclimation treatments. The results showed that mycorrhizal fungi were inoculated in the media after 7 days then sowed seeds in the media, the germination rate 73.2% was higher than other treatments. The best sowing density treatment and cultural media were 0.2 g•m-2 and sand mixed with cattle mature media, respectively. Above-mentioned propagated techniques of Spiranthes sinensis were transferred to industry to mass production seedlings.