文心蘭台農4 號-白雪微體繁殖與栽培技術之建立
Other Title
Establishment of Micropropagation System and Cultivation Tecchniques for Oncidium Tariflor Snow White
Start Page
86
End Page
97
ISBN
978-986-05-7182-0
Date Issued
2018-10
Date
2018-10
Author(s)
蔡東明
曹進義
陳威臣
夏奇鈮
莊耿彰
Tung-Ming Tsai
Chin-Yi Tsao
Uei-Chern Chen
Chi-Ni Hsia
Keng-Chang Chuang
Abstract
文心蘭台農4 號－白雪是我國第一個自行選育推廣的文心蘭切花品種，有別於市售的黃花品種，在顏色上是一個重大突破。經過北中南不同地區栽培環境、栽培技術及介質改良測試後，有些地區出現植株生長緩慢，不易增生新芽等問題。溫室試驗顯示栽培上施用氨蜜樂精或硝酸鉀，對白雪文心蘭生長具有促進作用；另外就本試驗與田間花農實際栽培測試，氨蜜樂精有助於新芽的增生。NPK 平均肥為一般文心蘭栽培最常用之肥料，資料顯示花寶2 號對假球莖肥大較有利，而花寶5 號高氮肥似乎對植株生長有促進效果。測試水苔、泥炭土、岩棉等不同栽培介質對白雪文心蘭生育性狀之影響，以水苔為介質對植株生長最有利，而岩棉對植株生長雖然較緩慢，但假球莖成長卻較為粗壯。微體繁殖方面，取文心蘭台農4 號-白雪花苞尚不明顯時之花梗為芽體誘導材料，花梗節培植體於0.6%次氯酸鈉水溶液消毒15 min 之效果較13 min 佳，汙染率為37%。花梗節培植體初次接種於含0.4 mg L-1 與0.1 mg L-1 NAA 之全量MS 培養基中可誘導出花梗和擬原球體 (protocorm-like bodies, PLBs) (15%)、花梗和營養芽 (32%)、花梗 (46%)以及似癒傷組織綠色團塊 (7%) 等四種芽體型態。PLBs 分別接種於不添加生長調節劑以及含與不含100 mlL-1 椰子水之不同鹽類濃度之花寶1 號培養基中，顯示以添加2g L-1 花寶1 號與100mlL-1 椰子水之HP2C 培養基較佳，其鮮重增加與增加倍數為7.1g 與6.1 倍。將子瓶階段之瓶苗 (3 cm explants) 於培養室以及水牆溫室環境下培養12 週，小苗生長皆良好，雖假球莖形成率、芽數、株高、葉數、葉長、葉寬、根數以及鮮重等性狀具顯著差異，但水牆溫室環境下培養之瓶苗植株較為精壯深綠，且根數與葉數較多，整體小苗品質較佳。
Oncidium Tainung No.4-Snow White is the first released cut-flower variety from government research unit in Taiwan. It is easy to distinguish the white flowers of Snow White from the yellow flowers of commemcial Oncidium varieties in the flower market. After different cultivation environments, cultivation techniques and medium improvement tests in different cultivation area, the plants grown in some areas showed the problems of slow growth and difficulty in accumulating new shoots. Use of fertilizers, AMILO and potassium nitrate, could promote the plant growth and new bud coming out on Oncidium Tainung No.4-Snow White. Hyponex No. 2 fertilizer was benefit for the growth of pseudobulb and Hyponex No. 5 was good for the growth of plant. For the use of culture media, Sphagnum moss was good for plant growth and rock wool was good for the pseudobulb enlarge. Nodal explants taken from flower stalks while bud invisible of Oncidium Tariflor Snow White were use as the materia for shoot induction. Result showed that nodal explants disinfecting with 0.6% sodium hypochlorite for 15 min with contamination rate 37% is better than disinfecting for 13 min (50%). Four types of shoot differentiation [flower stalk and protocorm like bodies (PLBs) (15%), flower stalk and vegetative shoot (32%), flower stalk (46%) and callus-like tissue (7%)] were induced when nodal explants were first inoculated on the MS medium containing 0.4 mg L-1 Benzyladenine (BA) and 0.1 mg L-1 1-Naphthaleneacetic. Different salt concentrations of Hyponex No. 1 (N:P:K=7:6:19) media which didn’t add with plant growth regulator (PGR) and with or without 100 mlL-1 coconut water were tested for optimal conditions of multiplication. Result showed that PLBs were best multiplied on 2 g L-1 Hyponex medium with 100 mlL-1 coconut water. PLBs average fresh weight increment was 7.1g and index fresh weight increase was 6.1.3 cm shoot as explants were inoculated in the final stage flask for 12 weeks growth well placed in pad and fan green house and tissue culture room conditions, respectively. Shoots in the final stage flask placed in pad and fan green house had more leaves and roots, and showed better quality with stronger and dark-green than flasks placed in tissue culture room, although percentage of shoot with pseudobulb, numbers of shoot, leaf and root, shoot height, leaf length, leaf width, and fresh weight traits were significantly better in tissue culture room.
Event(s)
強化台灣花卉產業競爭力之科研技術研討會
Proceedings of the Symposium on Agricultural Science and Technology Researches for Strengthening the Competitiveness of Flower Industry in Taiwan
Subjects
文心蘭
台農4號-白雪
肥培
介質
組織培養
Oncidium
Tainung No.4-Snow White
fertilization
culture medium
tissue culture
Publisher
農業試驗所
Type
book part
File(s)![Thumbnail Image]()
Loading...
Name
no209-9.pdf
Size
1.78 MB
Format
Adobe PDF
Checksum
(MD5):3186872dc73dc47f42cfdcbdd0bb1cf5