台灣地區咖啡果小蠹 (Hypothenemus hampei) 分離之Isaria javanica CAES1 鑑定與致病性測試
Other Title
Identification and Pathogenicity of Isaria javanica CAES1 Isolated from the Coffee Berry Borer (Hypothenemus hampei) in Taiwan
Journal
台灣農業研究
Journal Volume
69
Journal Issue
2
Pages
132-146
Date Issued
2020-06
Author(s)
Abstract
本研究自台灣咖啡田間感染真菌死亡的咖啡果小蠹 (Hypothenemus hampei) 上，分離到蟲生真菌CAES1，該菌株之菌落為白色至淡黃色或灰色，10–30℃均可生長，25℃菌絲生長速率最快。以光學顯微鏡檢視其無性產孢構造，其分生孢子梗分枝末端著生1–4 個瓶梗，瓶梗細胞長寬約3.7–5.0–6.4 μm × 1.9–3.0–3.5 μm，分生孢子串生，形狀呈橢圓形至長橢圓形，大小約為2.7–3.8–5.4 μm × 1.2–1.6–2.1 μm。以核糖體內轉錄區間 (internal transcribed spacer; ITS)、translation elongation factor-1α gene (TEF1) 及β-tubulin 的核酸序列以最大似然法 (maximum likelihood; ML) 進行親緣關係樹的重建分析，結果顯示本菌株於3 個基因所重建之親緣關係樹均歸類於Isaria javanica 的支系。An isolate of entomopathogenic fungi, CAES1, was isolated from a naturally infected coffee berry borer (Hypothenemus hampei; CBB) in a coffee field in Taiwan. Colonies of CAES1 were white to pale-yellow or grey. Mycelia could grow in range of 10℃ to 30℃, with the optimal temperature at 25℃, ranging from 10℃ to 30℃. The conidiophores formed branches with phialides in whorls of 1 to 4, with phialides 3.7–5.0–6.4 μm × 1.9–3.0–3.5 μm, and conidia forming in chain, 2.7–3.8–5.4 μm × 1.2–1.6–2.1 μm. CAES1 was identified as Isaria javanica based on morphology and phylogenetic analysis of the sequences of ribosomal internal transcribed spacer (ITS), translation elongation factor-1α (TEF1) and β-tubulin.
Subjects
咖啡果小蠹
Isaria javanica
台灣
生物防治
Coffee berry borer (Hypothenemus hampei)
Taiwan
Biological control
Publisher
農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
69-2-3.pdf
Size
2.77 MB
Format
Adobe PDF
Checksum
(MD5):ddd2f4df898b0e99244afc0b1ac34004