利用多倍體化及雜交親本篩選改進藍紫色蝴蝶蘭育種研究

曹進義; 陳威臣; 吳容儀; 吳姿穎; 夏奇鈮; Chin-Yi Tsao; Uei-Chern Chen; Rung-Yi Wu; Tzu-Ying Wu; Chi-Ni Hsia

doi:10.6156/JTAR.202006_69(2).0005

利用多倍體化及雜交親本篩選改進藍紫色蝴蝶蘭育種研究

Other Title

Polyploidization and Selection of Parents for Improving Breeding of Blue-Purple Phalaenopsis

Journal

台灣農業研究

Journal Volume

69

Journal Issue

2

Pages

154-168

Date Issued

2020-06

Author(s)

曹進義

陳威臣

吳容儀

吳姿穎

夏奇鈮

Chin-Yi Tsao

Uei-Chern Chen

Rung-Yi Wu

Tzu-Ying Wu

Chi-Ni Hsia

DOI

10.6156/JTAR.202006_69(2).0005

URI

https://scholars.tari.gov.tw/handle/123456789/15567

Abstract

本研究分析蝴蝶蘭花瓣組織之酸鹼值 (pH)，並據此篩選作為藍紫色蝴蝶蘭育種之雜交親本。此外，並以秋水仙素誘導藍紫色蝴蝶蘭種原之多倍體化，擴大藍紫色蝴蝶蘭種原在育種上之應用。以0.1 mM 秋水仙素浸漬處理藍紫色蝴蝶蘭原生種Phalaenopsis bellina fma. coerulea、Phalaenopsis equestris fma. coerulea 及藍紫色蝴蝶蘭栽培種Phalaenopsis Siam Treasure 無菌播種發芽後約6 wk 齡原球體 (protocorm) 8 d，進行多倍體誘導。結果顯示，3 種材料之原球體存活率在59.2–85.0% 之間，4 倍體誘導率為27.3–32.9%。比較秋水仙素誘導所得之4 倍體與2 倍體植株的園藝性狀，顯示4 倍體植株之花朵質地較硬實，且P. bellina fma. coerulea 與P. equestris fma. coerulea 之4 倍體花朵具較大花徑，4 倍體P. Siam Treasure 的花徑雖未明顯增大，但其花梗較粗短與2 倍體有明顯差異。4 倍體之雜交親和性，則需進一步測試。Polyploidy induction and selection of parents by pH of flower petal were used as strategies for improving breeding of blue-purple Phalaenopsis in this study. Using 6-wk-old protocorms of Phalaenopsis bellina fma. coerulea, Phalaenopsis equestris fma. coerulea and Phalaenopsis Siam Treasure as explants exposed in 0.1 mM colchicine for 8 d to induce polyploidy. Ploidy levels of seedlings derived from colchicine treatments were analyzed by flow cytometry and root tip staining. Results showed that survival rate among two species and one variety were 59.2–85.0%, and their tetraploid induction rates were 27.3–32.9%. Comparison of horticultural traits of diploid and tetraploid plants showed that all tetraploids had firmer petal texture than that of diploids. It was also found that tetraploids of P. bellina fma. coerulea and P. equestris fma. coerulea had larger flower diameter than that of their diploids. Although tetraploid plants of P. Siam Treasure had similar flower diameter with diploid, tetraploid plants had thicker and shorter stalk than their diploids.

Subjects

藍紫色蝴蝶蘭

矢車菊素

秋水仙素

倍體數

流式細胞儀

Blue-purple Phalaenopsis

Cyanidin

Colchicine

Polyploidy

Flow cytometry

Publisher

農業試驗所

Type

journal article

File(s)

Name

69-2-5.pdf

Size

2.88 MB

Format

Adobe PDF

Checksum

(MD5):a30edb59fd6f076d767de96c1f831d27