|Title:||利用多倍體化及雜交親本篩選改進藍紫色蝴蝶蘭育種研究||Other Titles:||Polyploidization and Selection of Parents for Improving Breeding of Blue-Purple Phalaenopsis||Authors:||曹進義
|Keywords:||藍紫色蝴蝶蘭;矢車菊素;秋水仙素;倍體數;流式細胞儀;Blue-purple Phalaenopsis;Cyanidin;Colchicine;Polyploidy;Flow cytometry||Issue Date:||Jun-2020||Publisher:||農業試驗所||Journal Volume:||69||Journal Issue:||2||Start page/Pages:||154-168||Source:||台灣農業研究||Abstract:||
本研究分析蝴蝶蘭花瓣組織之酸鹼值 (pH)，並據此篩選作為藍紫色蝴蝶蘭育種之雜交親本。此外，並以秋水仙素誘導藍紫色蝴蝶蘭種原之多倍體化，擴大藍紫色蝴蝶蘭種原在育種上之應用。以0.1 mM 秋水仙素浸漬處理藍紫色蝴蝶蘭原生種Phalaenopsis bellina fma. coerulea、Phalaenopsis equestris fma. coerulea 及藍紫色蝴蝶蘭栽培種Phalaenopsis Siam Treasure 無菌播種發芽後約6 wk 齡原球體 (protocorm) 8 d，進行多倍體誘導。結果顯示，3 種材料之原球體存活率在59.2–85.0% 之間，4 倍體誘導率為27.3–32.9%。比較秋水仙素誘導所得之4 倍體與2 倍體植株的園藝性狀，顯示4 倍體植株之花朵質地較硬實，且P. bellina fma. coerulea 與P. equestris fma. coerulea 之4 倍體花朵具較大花徑，4 倍體P. Siam Treasure 的花徑雖未明顯增大，但其花梗較粗短與2 倍體有明顯差異。4 倍體之雜交親和性，則需進一步測試。Polyploidy induction and selection of parents by pH of flower petal were used as strategies for improving breeding of blue-purple Phalaenopsis in this study. Using 6-wk-old protocorms of Phalaenopsis bellina fma. coerulea, Phalaenopsis equestris fma. coerulea and Phalaenopsis Siam Treasure as explants exposed in 0.1 mM colchicine for 8 d to induce polyploidy. Ploidy levels of seedlings derived from colchicine treatments were analyzed by flow cytometry and root tip staining. Results showed that survival rate among two species and one variety were 59.2–85.0%, and their tetraploid induction rates were 27.3–32.9%. Comparison of horticultural traits of diploid and tetraploid plants showed that all tetraploids had firmer petal texture than that of diploids. It was also found that tetraploids of P. bellina fma. coerulea and P. equestris fma. coerulea had larger flower diameter than that of their diploids. Although tetraploid plants of P. Siam Treasure had similar flower diameter with diploid, tetraploid plants had thicker and shorter stalk than their diploids.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.