|Title:||由 Corynespora cassiicola 引起之羅勒枝枯病||Other Titles:||Corynespora cassiicola Causes Dieback on Basil||Authors:||湯佳蓉
|Keywords:||羅勒;枝枯病;鐵刀木棒狀桿孢菌;Basil;Dieback;Corynespora cassiicola||Issue Date:||Sep-2021||Publisher:||農業試驗所||Journal Volume:||70||Journal Issue:||3||Start page/Pages:||196-205||Source:||台灣農業研究||Abstract:||
羅勒枝枯病 (basil dieback) 為羅勒栽培時期的真菌病害之一，於屏東縣萬丹鄉發現羅勒植株的枝條於採收後，從枝條傷口處開始乾枯，呈現黑褐色壞疽狀病徵，逐漸蔓延至全株、死亡。從不同羅勒栽培區，相同
病徵的罹病組織中分離出同一種真菌。室溫下，於馬鈴薯葡萄糖瓊脂 (potato dextrose agar; PDA) 培養 10 d 後，呈現邊緣為白色至淺灰色，中央為深橄欖綠色的菌落。根據此病原菌之型態特徵，與核醣體核酸基因 (rDNA)的內轉錄區 (internal transcribed spacer; ITS) 序列鑑定結果，將該病原菌鑑定為Corynespora cassiicola，並以柯霍氏法則證明，此真菌為造成羅勒枝枯病之病原菌。
Basil (Ocimum basilicum L.) is a perennial herb belonging to the family lamiaceae, and is commonly
cultivated as a condiment or spice in Taiwan. A symptom of dieback appeared on basil planted in the field and greenhouse at Wandan Township, Pingtung Country, and Luzhu Township, Kaohsiung City, Taiwan, during September 2019 to March 2020. The dark brown lesion initiated from the cutting ends made by the regular harvest, then extended progressively, and eventually resulted in the dieback of the branches and defoliation. Conidiophores and conidia characteristics of Corynespora cassiicola were observed from diseased tissues. Margin of lesion was excised and sterilized. Hyphae, once emerged from the plant tissue, were transferred from water agar, subcultured on potato dextrose agar
(PDA) plates, and incubated at room temperature.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
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