Abscisic acid promotes development of somatic embryos on converted somatic embryos of Corydalis yanhusuo (Fumariaceae)

Chao-Lin Kuo; Abhay P. Sagare; Shu-Fung Lo; Chen-Yue Lee; Chung-Chuan Chen; Hsin-Sheng Tsay

doi:10.1078/0176-1617-00740

Abscisic acid promotes development of somatic embryos on converted somatic embryos of Corydalis yanhusuo (Fumariaceae)

Journal

Journal of Plant Physiology

Journal Volume

159

Journal Issue

4

Pages

423-427

Date Issued

2002-04

Author(s)

Chao-Lin Kuo

Abhay P. Sagare

Shu-Fung Lo

Chen-Yue Lee

Chung-Chuan Chen

Hsin-Sheng Tsay

DOI

10.1078/0176-1617-00740

URI

https://www.sciencedirect.com/science/article/pii/S0176161704702485?via%3Dihub

https://scholars.tari.gov.tw/handle/123456789/16474

Abstract

Converted somatic embryos of Corydalis yanhusuo showed direct development of somatic embryos in the region at the junction of cotyledonary leaf and root when cultured on Murashige and Skoog (MS) basal medium supplemented with 0-16 mg/L abscisic acid (ABA) and with 2 mg/L being optimum for further development. Histological and scanning electron microscopic studies confirmed the direct origin of embryos from the epidermal layer of cells. The use of liquid MS basal medium with 0.1 mg/L gibberellic acid (GA(3)) facilitated embryo conversion within fifteen days of culture. Phenotypically normal plants were recovered from converted somatic embryos and the plants showed well-developed tubers and in vitro flowering after 4 months of culture.

Subjects

ABA

corydalis yanhusuo

in vitro flowering

medicinal plant

papaveraceae

plant propagation

Publisher

Elsevier GMbh

Type

journal article