|Title:||Abscisic acid promotes development of somatic embryos on converted somatic embryos of Corydalis yanhusuo (Fumariaceae)||Authors:||Chao-Lin Kuo
Abhay P. Sagare
|Keywords:||ABA;corydalis yanhusuo;in vitro flowering;medicinal plant;papaveraceae;plant propagation||Issue Date:||Apr-2002||Publisher:||Elsevier GMbh||Journal Volume:||159||Journal Issue:||4||Start page/Pages:||423-427||Source:||Journal of Plant Physiology||Abstract:||
Converted somatic embryos of Corydalis yanhusuo showed direct development of somatic embryos in the region at the junction of cotyledonary leaf and root when cultured on Murashige and Skoog (MS) basal medium supplemented with 0-16 mg/L abscisic acid (ABA) and with 2 mg/L being optimum for further development. Histological and scanning electron microscopic studies confirmed the direct origin of embryos from the epidermal layer of cells. The use of liquid MS basal medium with 0.1 mg/L gibberellic acid (GA(3)) facilitated embryo conversion within fifteen days of culture. Phenotypically normal plants were recovered from converted somatic embryos and the plants showed well-developed tubers and in vitro flowering after 4 months of culture.
|Appears in Collections:||SCI期刊|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.