|Title:||Identification of Differentially Expressed Genes during Initial Floral Development in Oncidium Pseudobulb||Other Titles:||花序發育初期於文心蘭假球莖中具差異性表現基因之選殖||Authors:||Chih-Yu Wang
|Keywords:||Oncidium Gower Ramsey;Suppression subtractive hybridization (SSH);Microarray;Flower initiation-associated genes;Pseudobulb;文心蘭南西品種;抑制扣除雜交;微陣列;開花誘導相關基因;假球莖||Issue Date:||Jun-2007||Publisher:||中華農藝學會、中華農業氣象學會||Journal Volume:||4||Journal Issue:||2||Start page/Pages:||135-144||Source:||作物、環境與生物資訊||Abstract:||
We used suppression subtractive hybridization (SSH) and microarray techniques to isolate genes that increased abundantly in the pseudobulb of Oncidium Gower Ramsey during initial inflorescence development. A subtraction cDNA library was generated by using the cDNA from pseudobulbs in two different stages: the ones with initial inflorescence (2-3 cm) and the other with longer inflorescence (7-8 cm), and the former was subtracted against the latter. In this work, a total of 425 clones with differential expression were obtained. With microarray analysis, forty-five clones which have expression levels up regulated two-fold in the initial inflorescence stage were obtained, and they shared the average insert size of 328 bp. Thirty-one ESTs showed homology to the known genes. Eight out of 31 genes were related to stress responses, including peroxidase, mannosebinding lectin precursor, metallothionein, and RD22. It is interesting that the appearance of peroxidase gene related to flowering time. Ten genes were involved in regulation, including latex-like protein, dicarboxylate transporter, profilin, allantoinase, ADP-ribosylation factor, and heat shock protein. One gene was related to cell wall metabolism, which is GTP-binding protein Rac. One gene encoded a structural protein, alpha-tubulin. Four genes were involved in protein synthesis and degradation, including ribosomal protein and ubiquitin. Seven genes were related to photosynthesis. Additionally, fourteen genes showed no homology with previously-reported sequences.
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