Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae) - an important medicinal herb

Chung-Chuan Chen; Suh-Jau Chen; Abhay P. Sagare; Hsin-Sheng Tsay

Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae) - an important medicinal herb

Journal

Botanical Bulletin of Academia Sinica

Journal Volume

42

Journal Issue

1

Pages

1-7

Date Issued

2001-01

Date

2001-01

Author(s)

Chung-Chuan Chen

Suh-Jau Chen

Abhay P. Sagare

Hsin-Sheng Tsay

URI

https://scholars.tari.gov.tw/handle/123456789/1778

https://ejournal.sinica.edu.tw/bbas/toc/issue-2001-1.html

Abstract

Efficient plant regeneration system has been established from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae), an important medicinal herb. Adventitious shoots were induced by culturing the stem internode explants on Murashige and Skoog (MS) basal medium supplemented with 2.22-35.51 µM N6-benzylaminopurine (BA) in combination with 0.54 µM a-naphthaleneacetic acid (NAA). The regeneration potential varied with the developmental stage of the stem explant and growth regulator combination. Adventitious shoots formed a new crop of multiple shoots when subcultured on MS medium supplemented with 17.75 µM BA. Shoots were rooted on 1/4-strength MS basal medium supplemented with 5.37 µM NAA. Plantlets produced from shoots were transferred to soil and acclimatized in a growth chamber. Plants appearing morphologically normal were obtained six to seven months after culture of stem internode explants.

Subjects

Adenophora triphylla (Thunb.) A. DC.

Campanulaceae

De novo regeneration

Germplasm conservation

Medicinal herb

Micropropagation

Sha shen

Publisher

Institute of Botany, Academia Sinica

Type

journal article