Formation of Protoberberine-Type Alkaloids by the Tubers of Somatic Embryo-Derived Plants of Corydalis yanhusuo

Journal
Planta Medica
Journal Volume
67
Journal Issue
9
Pages
839-842
Date Issued
2001-12
Date
2001-12
Author(s)
Ya-Lin Lee
Abhay P. Sagare
Chen-Yue Lee
Hai-Tung Feng
Yen-Chen Ko
Jei-Fu Shaw
Hsin-Sheng Tsay
DOI
URI
Abstract
The effects of 0.5 - 5 mg/l abscisic acid [ABA], 0.5 - 10 mg/l (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol [paclobutrazol] and 0.5 - 2 mg/l α-cyclopropyl-α-(4-methoxyphenyl)-5-pyrimidinemethanol [ancymidol], 0.5 - 5 mg/l gibberellic acid [GA3] and 15 - 100 mg/l polyethylene glycol [PEG] 4000 supplemented in half-strength Murashige and Skoog's (MS) medium on the production of the two major protoberberine-type alkaloids (D,L-tetrahydropalmatine and D-corydaline) by the tubers of somatic embryo-derived plants of Corydalis yanhusuo were examined. Somatic embryo derived plants were also maintained for 6 months on half-strength MS medium containing 0.1 mg/l GA3 or 0.5 mg/l paclobutrazol. The alkaloid contents were determined by high performance liquid chromatography (HPLC). The analysis revealed that the contents of D,L-tetrahydropalmatine and D-corydaline in the tubers of somatic embryo-derived plants were higher than the marketed crude drug and varied with growth regulator/PEG-4000 treatment and the age of the plant.
Subjects
D-Corydaline
Corydalis yanhusuo
Fumariaceae
HPLC
protoberberine alkaloids
D,L-tetrahydropalmatine
tissue culture
yanhusuo
Publisher
Georg Thieme Verlag
Type
journal article
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