Profiling and Characterization Antioxidant Activities in Anoectochilus formosanus Hayata

Journal
Journal of Agricultural and Food Chemistry
Journal Volume
50
Journal Issue
7
Pages
1859-1865
Date Issued
2002-03
Date
2002-03
Author(s)
Sheng-Yang Wang
Yueh-Hsiung Kuo
Hsing-Ning Chang
Pei-Ling Kang
Hsin-Sheng Tsay
Ku-Feng Lin
Ning-Sun Yang
Lie-Fen Shyur
DOI
URI
Abstract
Phytochemical characteristics and antioxidant activities of the crude and fractionated plant extracts of Anoectochilus formosanus were evaluated using five different assay systems. An acid-treatment (2 N HCl in 95% ethanol) was employed to treat a butanol fraction (BuOH), creating an acid-hydrolyzed BuOH fraction. The IC50 values for DPPH radicals in the BuOH and acid-hydrolyzed BuOH fractions were 0.521 and 0.021 mg/mL, respectively. The acid-hydrolyzed BuOH exhibited approximately 5-fold higher activity in scavenging superoxide anion than catechin. The acid-hydrolyzed BuOH fraction also effectively protected φ x174 supercoiled DNA against strand cleavage induced by H2O2 and reduced oxidative stress in HL-60 cells. Metabolite profiling showed that the aglycones of flavonoid glycosides in BuOH were produced after acid hydrolytic treatment, and this resulted in a significant increase in antioxidant activities of acid-hydrolyzed BuOH. One new diarylpentanoid, kinsenone, and three known flavonoid glycosides and their derivatives were identified for the first time from A. formosanus, with strong antioxidant properties.
Subjects
Anoectochilus formosanus
antioxidant activity
acid treatment
flavonoid glycosides
Publisher
American Chemical Society
Type
journal article
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