|Title:||Propagation of Haemaria discolor via in vitro seed germination||Authors:||Y.-J. Shiau
S. M. Nalawade
|Keywords:||in vitro propagation;Jewel orchid;pollination||Issue Date:||Sep-2005||Publisher:||Academy of Sciences of the Czech Republic||Journal Volume:||49||Journal Issue:||3||Start page/Pages:||341-346||Source:||Biologia Plantarum||Abstract:||
In vitro propagation protocol for Haemaria discolor (Ker) Lindl. var. dawsoniana by artificial cross-pollination and asymbiotic germination of seeds has been developed. Fruit set (100 %) was obtained when the pollinia and ovules of various aged flowers were used for pollination. In vitro germination of seeds obtained from capsules of various ages was achieved on half-strength Murashige and Skoog’s (MS) medium supplemented with 3 % sucrose and 0.85 % agar. The germinated seedlings were cultured on half-strength MS medium with 0.2 % activated charcoal, 8 % banana homogenate, 0.1 mg dm−3 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ) and 1 mg dm−3 α-naphthaleneacetic acid (NAA). Ninety-six percent of plantlets survived after hardening in greenhouse.
|Appears in Collections:||SCI期刊|
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