Linkage of restriction fragment length polymorphisms and isozymes in Citrus
Resource
Theoretical and Applied Genetics
Journal
Theoretical and Applied Genetics
Journal Volume
84
Journal Issue
1-2
Pages
39-48
Date Issued
1992-06
Date
1992-06
Author(s)
R. E. Durham
P. C. Liou
F. G. Gmitter Jr.
G. A. Moore
Abstract
Genetic linkage analysis was performed using two segregating populations of citrus. One population arose from an intergeneric backcross of Citrus grandis (L.) Osb. cv ‘Thong Dee’ and Poncirus trifoliata (L.) Raf. cv ‘Pomeroy’, using the former as the recurrent (female) parent. The other population came from an interspecific backcross of C. reticulata Blanco cv ‘Clementine’ and C. x paradisi Macf. cv ‘Duncan’, using the former as the recurrent (male) parent. A total of 11 isozyme and 58 restriction fragment length polymorphisms were found to segregate in a monogenic fashion in one or both populations. Linkage analysis revealed that 62 of the loci examined mapped to 11 linkage groups, while 7 loci segregated independently from all other markers. Gene order was highly conserved between the maps generated from the two divergent segregating populations. Possible applications of the use of such maps in tree fruit breeding are discussed.
Subjects
Citrus spp.
Fruit breeding
Genetic map
Molecular markers
RFLP
Publisher
Springer-Verlag
Type
journal article