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  2. 1.總所研究單位
  3. (2)遺傳資源及生物技術組
Please use this identifier to cite or link to this item: https://scholars.tari.gov.tw/handle/123456789/18233
Title: 良質米研究團隊-水稻基因功能評估平臺之建立
Authors: 林大鈞 
賴慧真
王怡雯 
謝曉鶯
Keywords: 代謝工程;類黃酮生合成途徑;異黃酮;35S啓動子;基因轉殖;metabolic engineering;flavonoids biosynthetic pathway;isoflavone;35S promoter;transformation
Issue Date: 17-Oct-2012
Publisher: 行政院農業委員會臺中區農業改良場
Related Publication(s): 臺中區農業改良場特刊第115號
Start page/Pages: 129-142
Source: 良質米研究團隊研發成果研討會專輯 
Conference: 良質米研究團隊研發成果研討會
Abstract: 
水稻基因功能評估平臺已建立一轉殖效率約15%的TNG67轉殖流程,亦完成S35、玉米Ubi1-1、GluC及GluB5啓動子驅動螢光基因(AcGFP及ZsGFP)等表現系統的構築,經轉形至Agrobacterium tumefaciens EHA105後,即以農桿菌媒介轉形法,將螢光基因導入TNG67水稻。
A TNG67 transgenic procedure with effciency of 15% has been establish in this study. At the same time, the expression systems for the green fluorescent genes, AcGFP and ZsGFP, under the control of 35S, maize Ubi-1 or endosperm specific promoters, GluB5 or GluC, also have been constructed and transformed to TNG67 successfully.
URI: https://www.tdais.gov.tw/ws.php?id=8186
https://scholars.tari.gov.tw/handle/123456789/18233
ISBN: 978-986-03-6908-3
Appears in Collections:(2)遺傳資源及生物技術組

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