https://scholars.tari.gov.tw/handle/123456789/18233| Title: | 良質米研究團隊-水稻基因功能評估平臺之建立 | Authors: | 林大鈞 賴慧真 王怡雯 謝曉鶯 |
Keywords: | 代謝工程;類黃酮生合成途徑;異黃酮;35S啓動子;基因轉殖;metabolic engineering;flavonoids biosynthetic pathway;isoflavone;35S promoter;transformation | Issue Date: | 17-Oct-2012 | Publisher: | 行政院農業委員會臺中區農業改良場 | Related Publication(s): | 臺中區農業改良場特刊第115號 | Start page/Pages: | 129-142 | Source: | 良質米研究團隊研發成果研討會專輯 | Conference: | 良質米研究團隊研發成果研討會 | Abstract: | 水稻基因功能評估平臺已建立一轉殖效率約15%的TNG67轉殖流程，亦完成S35、玉米Ubi1-1、GluC及GluB5啓動子驅動螢光基因(AcGFP及ZsGFP)等表現系統的構築，經轉形至Agrobacterium tumefaciens EHA105後，即以農桿菌媒介轉形法，將螢光基因導入TNG67水稻。 A TNG67 transgenic procedure with effciency of 15% has been establish in this study. At the same time, the expression systems for the green fluorescent genes, AcGFP and ZsGFP, under the control of 35S, maize Ubi-1 or endosperm specific promoters, GluB5 or GluC, also have been constructed and transformed to TNG67 successfully. |
URI: | https://www.tdais.gov.tw/ws.php?id=8186 https://scholars.tari.gov.tw/handle/123456789/18233 |
ISBN: | 978-986-03-6908-3 |
| Appears in Collections: | 生物技術組 |
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