Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure)

H.S. Tsay; C.C. Yeh; J.Y. Hsu

doi:10.1007/BF00232396

Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure)

Resource

Plant Cell Reports 9(7): 349-351

Journal

Plant Cell Reports

Journal Volume

9

Journal Issue

7

Pages

349-351

Date Issued

1990-11

Date

1990-11

Author(s)

H.S. Tsay

C.C. Yeh

J.Y. Hsu

DOI

10.1007/BF00232396

URI

https://scholars.tari.gov.tw/handle/123456789/1834

https://link.springer.com/article/10.1007/BF00232396

Abstract

Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of response for callus induction. Prolonged culture of these embryogenic calli on the original medium or subculture to an auxin-free medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. Chromosome counts from root-tip cells of anther-derived plant indicated that they were haploid (N=36).

Subjects

Sucrose

Agar

Charcoal

Plant Regeneration

Callus Induction

Publisher

Springer

Type

journal article

File(s)

Name

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Size

21.96 KB

Format

HTML

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