Surveillance of Rice Blast Resistance Effectiveness and Emerging Virulent Isolates in Taiwan

Jauhar Syauqi; Rong-Kuen Chen; An-Hsiu Cheng; Yea-Fang Wu; Chia-Lin Chung; Chun-Chi Lin; Hau-Ping Chou; Hsin-Yuh Wu; Jen-You Jian; Chung-Ta Liao; Chien-Chih Kuo; Sheng-Chi Chu; Yi-Chen Tsai; Dah-Jing Liao; Yong-Pei Wu; Abdul Latief Abadi; Liliek Sulistyowati; Wei-Chiang Shen

doi:10.1094/PDIS-12-21-2806-RE

Surveillance of Rice Blast Resistance Effectiveness and Emerging Virulent Isolates in Taiwan

Journal

Plant Disease

Journal Volume

106

Journal Issue

12

Pages

3187-3197

Date Issued

2022-12

Author(s)

Jauhar Syauqi

Rong-Kuen Chen

An-Hsiu Cheng

Yea-Fang Wu

Chia-Lin Chung

Chun-Chi Lin

Hau-Ping Chou

Hsin-Yuh Wu

Jen-You Jian

Chung-Ta Liao

Chien-Chih Kuo

Sheng-Chi Chu

Yi-Chen Tsai

Dah-Jing Liao

Yong-Pei Wu

Abdul Latief Abadi

Liliek Sulistyowati

Wei-Chiang Shen

DOI

10.1094/PDIS-12-21-2806-RE

URI

https://apsjournals.apsnet.org/doi/10.1094/PDIS-12-21-2806-RE

https://scholars.tari.gov.tw/handle/123456789/18639

Abstract

Rice blast caused by Magnaporthe oryzae is a dangerous threat to rice production and food security worldwide. Breeding and proper deployment of resistant varieties are effective and environmentally friendly strategies to manage this notorious disease. However, a highly dynamic and quickly evolved rice blast pathogen population in the field has made disease control with resistance germplasms more challenging. Therefore, continued monitoring of pathogen dynamics and application of effective resistance varieties are critical tasks to prolong or sustain field resistance. Here, we report a team project that involved evaluation of rice blast resistance genes and surveillance of M. oryzae field populations in Taiwan. A set of International Rice Research Institute-bred blast-resistant lines (IRBLs) carrying single blast resistance genes was utilized to monitor the field effectiveness of rice blast resistance. Resistance genes such as Ptr (formerly Pita2) and Pi9 exhibited the best and most durable resistance against the rice blast fungus population in Taiwan. Interestingly, line IRBLb-B harboring the Pib gene with good field protection has recently shown susceptible lesions in some locations. To dissect the genotypic features of virulent isolates against the Pib resistance gene, M. oryzae isolates were collected and analyzed. Screening of the AvrPib locus revealed that the majority of field isolates still maintained the wild-type AvrPib status but eight virulent genotypes were found. Pot3 insertion appeared to be a major way to disrupt the AvrPib avirulence function. Interestingly, a novel AvrPib double-allele genotype among virulent isolates was first identified. Pot2 repetitive element-based polymerase chain reaction (rep-PCR) fingerprinting analysis indicated that mutation events may occur independently among different lineages in different geographic locations of Taiwan. This study provides our surveillance experience of rice blast disease and serves as the foundation to sustain rice production.

Subjects

AvrPib

field resistance

IRRI-bred blast-resistant lines

Magnaporthe oryzae

rice blast

Publisher

American Phytopathological Society

Type

journal article