Genetically Engineering Citrus with the Insecticidal Gene from Bacillus Thuringiensis Subsp. Tenebrionis

Abstract

Genetic transformation of Citrus reticulata Blanco cv.‘Ponkan’ mandarin was carried out by co-culturing freshlyexcised nucelli with Agrobacterium tumefacients that harboreda plasmid containing the Coleoptera specific insecticidalgene from B. thuringiensis ssp. 椪柑 (Citrus reticulata Blanco cv. Ponkan mandarin ) 之抗甲蟲基因轉殖研究是將幼果中之未成熟胚珠，切取其珠心組織與俱抗甲蟲基因之冠瘤菌共同培養，藉細菌感染植物細胞之過程使抗蟲基因自然植入植物細胞內達到基因轉殖效果。