利用染色體操縱法改良小麥蛋白質含量及胺基酸組成之研究

Abstract

本研究系利用小麥畸多元體 (aneuploid) 檢討如何操縱染色體 (chromosome manipulation)，以改良蛋白質之含量及胺基酸之組成。茲將主要試驗結果摘要如下： 以 Udy 氏之 DBC 法所分析小麥蛋白質之平均含量為 15.18±1.95%，較以 Kjeldahl法分析所得結果 14.43±1.81%稍高；但經 t 測驗結果，兩者之差異不顯著。二法所得蛋白質含量之相關系數高達 r=0.950，表示 DBC 法之速簡蛋白質分析結果甚為可靠。 The present experiment deals with the nutritional improvement of wheat through manipulation of chromosomes by using various aneuploid wheats. The results obtained are summarized in the followings: 1.Protein contents was measured by Udy's Dye-Binding-Capacity (DBC) method as well as Kjeldahl's method. The mean percent protein obtained by DBC method was a little higher (15.186±1.95%) than that obtained by the Kjeldahl method (14.43±1.811%), however, the difference was statistically insighificant. Correlation coefficient of percent protein obtained by the two methods was very ight (r = 0.950) indicating that the use of the quick DBC method for protein analysis had been quite reliable.