臺灣金線連之組織培養(Ⅲ.)種子成熟度及前處理對萌芽與幼苗發育之影響
Other Title
Tissue Culture of Anoectochilus formosanus Hayata(Ⅲ.) The Influence of Seed Maturity and Pretreatment on Seed Germination and Seedling Development
Journal
中華農學會報
Journal Issue
新183
Pages
69-78
Date Issued
1998-09
Author(s)
Abstract
臺灣金線連輕人工授粉所得不同成熟度果莢之種子，播種於含1/2MS基本鹽類、3% 蔗糖及0.9% agar之固體培養基，經培養60天後調查發現，87.5%果莢成熟度之種子萌芽率 最高可達51%，100%成熟度者次之為37%，低於75%成熟度者均不具發芽能力；87.5%成 熟度果莢之種子，播種前以不經3% H2O2溶液預處理較佳；野生台灣金線連自然授粉之 成熟果莢，以5±1℃冷藏，可至少保存21天而不影響種子萌芽率；相同冷藏條件處理種子， 則至少可保存15天；若將種子置於25℃室溫下，則壽命僅能維持2天，顯示冷藏處理可有 效延長台灣金線連種子壽命；比較臺灣金線連人工授粉與自然授粉種子之萌芽率發現，自然 授粉產生之種子之萌芽率86%比人工授粉之51%明顯較高，顯示人工授粉技術尚有待改進。
Capsules of Anoectochilus formosanus Hayata at various stage of maturity were collected and a germination test was proceeded on a medium containing 1/2 MS basic salts with 3% sucrose and 0.9% agar. The results showed that the capsules in 87.5% maturity had the highest germination rate (51%), and the full mature capsules had 37% germination rate, however seeds maturity that below 75% did not germinate. Pretreatment the seeds (87.5% in maturity) with 3% H2O2 was not necessary since it might be detrimental to embryos and rhizome growth. Cold storage of seeds or capsules at 5±1℃ and 100% humidity was useful to prolong seed vitality to 15-21 days. Storage seeds at room temperature (25℃) for 2 days was detrimental to the viability of embryos. A lower germination rate of seeds by hand pollination then the seeds from insect pollination was obtained in this study.
Subjects
臺灣金線連
組織培養
種子萌芽率
anoectochilus formosanus hayata
tissue culture
seed germination
Publisher
中華農學會
Type
journal article