利用蘇力菌殺蟲晶體蛋白基因育成抗蟲植物之研究
Other Title
Breeding for Insect Tolerant Transgenic Plants by Using Insecticidal Crystal Protein Genes form Bacillus thuringiensis
Journal
作物之遺傳育種及生理栽培硏討會專集(I)
Pages
141-142
Date Issued
1993-06
Author(s)
陳鵬文
曾經洲
高穗生
蔡新聲
陳良築
Pen-Wen Chen
Ching-Chou Tzeng
Suey-Sheng Kao
Hsin-Sheng Tsay
Liang-Jwu Chen
Abstract
本試驗利用聚合脢連鎖反應的技術，自六個來源不同的蘇力菌種中，分離出多個殺蟲昌體蛋白基因。這些自聚合脢連鎖反應所分離的殺蟲晶體蛋白基因，經選殖後得到含蘇力菌殺蟲昌體蛋白基因的表現載體。這此表現載體，僅含殺蟲晶體蛋白基因的N端1至652胺基酸的毒蛋白基因部份，與載體的lac Z 基因可形成融合蛋白質。
In the study, six Bt-icp (Insecticidal crystal protein of Bacillus thuringiensis) genes were isolated from various B. thuringiensis strains by PCR techniques. The PCR amplified Bt-icp gene contains only the first 652 amino acids, the N-terminal half toxic domain, of the intact Bt-icp gene. To ensure the proper expression of the amplified Bt-icp genes in E. coli and plants, one start and one stop codon were incorporated into the amplified DNA fragment by creating the NcoI and SstI restriction sites respectively in the PCR primers.
Event(s)
臺中場特刊第30號
Subjects
蘇力菌
有毒基因
轉殖植物
bacillus thuringiensis
toxin gene
transgenic plants
Publisher
臺中區農業改良場
Type
conference paper