探討基因轉殖水稻稻稈殘體對土壤微生物相的影響
Other Title
Exploring the Impact of Transgenic Rice Straw Decomposition on Soil Microbial Composition
Journal
台灣農業研究
Journal Volume
72
Journal Issue
4
Pages
333-342
Date Issued
2023-12-04
Author(s)
Abstract
為瞭解過量表達植酸酵素之轉殖水稻 (AAN) 稻稈殘體掩埋土中後，對土壤微生物相之影響，本研究進行為期1 年的監測，追蹤掩埋期間土壤微生物相的變化。結果顯示稻稈殘體經180 d 掩埋，對照品種非轉殖‘TNG 67’ (Oryza sativa L. ssp. japonica cv. Tainung 67; ‘TNG 67’) 水稻掩埋土壤中的總細菌數，顯著高於AAN轉殖系，隨著時間增加，兩者之總細菌數皆有下降趨勢。土壤總真菌數隨著掩埋時間而變化較大，雖然皆以AAN 轉殖系較高，但120 d 後與 ‘TNG 67’ 相較兩者差異不顯著，且AAN 轉殖系的總真菌數在360 d 時有較明顯之下降。土壤放射線菌數方面，‘TNG 67’ 對照水稻隨著時間緩慢上升，AAN 轉殖系則在120 d 後菌數才明顯增加，且兩者皆在300 d 之後開始下降，至360 d 時二者無顯著差異。使用變性梯度膠體電泳分析，結果顯示AAN 轉殖系周邊土壤菌相數量增加，在180 d 時AAN 轉殖系與 ‘TNG 67’ 水稻之細菌菌群相似度高同屬一群；在360 d 時AAN 轉殖系與 ‘TNG 67’ 之細菌菌群亦顯示同屬一群。綜合平板計算法與變性梯度膠體電泳模式之結果，推測在殘體掩埋初期，AAN 轉殖系與 ‘TNG 67’ 之殘體，在土壤中之微生物數量與細菌多樣性方面有較高之差異性，但這些差異在掩埋達360 d 後逐漸減少並趨向一致。
To understand the impact of buried rice straw residues of transgenic rice (AAN), which overexpresses phytase enzymes on the soil microbial community, a year-long monitoring experiment was conducted to track changes in the soil microbial composition. The results revealed that after 180 d of burial, the total bacterial count in the non-transgenic control variety ‘TNG 67’ (Oryza sativa L. ssp. japonica cv. Tainung 67; ‘TNG 67’) was significantly higher than that of the AAN transgenic line, and a declining trend was found along with time for both varieties. The total fungal count in the soil exhibited greater variability along with burial time. Although the AAN transgenic line generally had higher fungal counts, the difference between the two was not statistically significant after 120 d of burial. Notably, the total fungal count in the AAN transgenic line decreased significantly at 360 d. Regarding actinomycetes in the soil, the total count gradually increased over time in the ‘TNG 67’ control rice. However, an obvious increase was found in the AAN transgenic line after 120 d decomposition. Nevertheless, counts of both varieties began to decline after 300 d, and by 360 d, there was no significant difference between the two. Analysis using denaturing gradient gel electrophoresis (DGGE) showed an increased abundance of microbial communities near the AAN transgenic line. However, the bacterial communities derived from the AAN transgenic line and ‘TNG 67’ rice were found in the same cluster with high similarity at 180 d. The same result was found at 360 d in which the AAN transgenic line and ‘TNG 67’ bacterial communities exhibited similarity within the same cluster. Integrating the results from plate counting and DGGE analysis suggests that during the early stages of straw burial, there were notable differences in microbial count and bacterial diversity between the AAN transgenic line and the ‘TNG 67’ control. However, these differences gradually diminished or converged after 360 d of burial.
Subjects
水稻
基因轉殖作物
土壤微生物
稻稈
rice
transgenic crop
Soil bacterial community
rice straw
Publisher
農業試驗所
Type
journal article
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