Virus-tested Lycoris aurea plants from apical meristems of adventitiously regenerated shoots
Other Title
金花石蒜試管內之不定芽再生莖其生長點培養建立無病毒健康苗
Journal
Botanical Studies
Journal Volume
47
Journal Issue
2
Pages
163-166
Date Issued
2006-04
Author(s)
Li-Chun HUANG
Wha-Shin HSU
You-Ming CHANG
Pan-Chi LIOU
Bau-Lian HUANG
Chin-An CHANG
黃麗春
劉邦基
許華欣
黃寶蓮
張清安
Abstract
Adventitious shoots were first regenerated in vitro from twin-scale sections of virus-infected Lycoris aurea Herb. cv. ‘Golden spider lily’. Their apical meristems were then excised and cultured to redevelop shoots. Explants were not larger than 0.1 mm and were limited to the dome and subjacent stem, without leaf primordia. The redeveloped shoots were multiplied, rooted, ardened, and acclimatized, before transfer to an insect-free greenhouse. ELISA assays were performed for three lycoris potyviruses (lycoris potyvirus, LPV; lycoris mild mottle virus, LyMMV; and lycoris virus Taiwan, LVT) and for cucumber mosaic virus (CMV). Assays after one year and re-assays in the subsequent three years revealed no LyMMV, LPV, or CMV in the meristem-derived plants or in their clonal progeny. The virus-tested plants are now being increased as foundation stock.
金花石蒜又名金色蜘蛛百合為台灣原生花卉，本研究先以試管內雙鱗片培養建立試管再生系統，誘導不定芽大量分化和繁殖，不定芽長大成苗後再切取其小於 0.1 毫米高之微小莖頂生長點（不帶有葉原體），促使其發育抽長成莖、及繁殖、發根、馴化後，移植管外於隔離溫室繼續生長茁壯。以酵素抗体分析法（ELISA）鑑定種植一年後及連續四年共五年追蹤四種金花石蒜 potyviruses 病毒（LPV、LyMMV 和 LVT）及胡瓜嵌紋病毒（CMV），確定無上述四種病毒，即無 LPV、LyMMV、LVT、CMV 之毒素病存在。自莖頂生長點培養獲得之健康無性系，現已成為原原種（Fundation stock）建立大量田間種植及繁殖。
Subjects
cucumber mosaic virus
lycoris mild mottle virus
Lycoris potyvirus
Lycoris virus Taiwan
lycoris
meristem culture
Virus tested plants
Publisher
Academia Sinica
Type
journal article