Effects of Sweet Potato Leaf Extracts and Chlorogenic Acid on Glucose Uptake in C2C12 Cells
Journal
Agronomy
Journal Volume
14
Journal Issue
12
Start Page
2855
Date Issued
2024-12
Author(s)
by Kuan-Hung Lin
Wen-Xin Chu
Yu-Tsung Lee
Yi-Hung Li
Wei-Tang Chang
Yi-Ping Yu
Ming-Chih Shih
Yung-Chang Lai
Chun-Ping Lu
Pi-Yu Chao
Abstract
Edible sweet potato leaf can be exploited in the management and treatment of insulin resistance. This study investigated the effects of three sweet potato leaf extracts (SPLEs) and chlorogenic acid (CGA) on glucose uptake (2-NBDG uptake and GLUT4 abundance) and expression of their related regulatory factors (such as IR, IRS-1, p-Akt1, and p-AMPKα1 abundances) using Western blot analysis in insulin-treated insulin-resistant C2C12 cells. The results show that both purple and green SPLEs improved glucose (2-NBDG) uptake efficacy with insulin treatments, and both SPLEs also increased GLUT4 and IR abundances via activation of p-Akt in the PI3K/Akt pathway, whereas the IR abundance efficacy influence was the same as in the insulin-treated group. The yellow SPLE and CGA have higher protein abundances of IR and IRS-1, while CGA (20 μg/mL) exhibits the highest abundance of p-Akt1 and p-AMPKα1. These results suggest potential benefits of purple and green SPLEs in promoting glucose uptake, possibly through modulation of insulin signaling pathways.
Subjects
sweet potato leaf
insulin resistance
chlorogenic acid
2-NBDG
GLUT4
PI3K/Akt pathway
type 2 diabetes
SDGs
Publisher
MDPI
Description
Ministry of Science and Technology, Taiwan
Type
journal article