Effect of Temperature on the Mycelium Growth and Sporulation of Four Pathotypes of Pyricularia oryzae in Taiwan
Other Title
溫度對臺灣稻熱病菌 (Pyricularia oryzae) 四種病原型菌株生長及產孢之影響
Journal
台灣農業研究
Journal Volume
74
Journal Issue
2
Start Page
161
End Page
175
ISSN
2790-086X
Date Issued
2025-06-06
Author(s)
Abstract
Climate change-related variations in temperature and precipitation may impact the ecology of certain plant pathogens and vectors that are sensitive to changes in humidity and temperature, which could, in turn, affect the incidence and temporal and spatial distribution of plant diseases. Previous re-search has shown that the rice blast fungus in Taiwan could be divided into 5 pathotype clusters based on virulence to Lijiangxintuanheigu monogenic lines (LTH MLs), with differences in habitat tem-peratures between these clusters. In this work, we investigated the effect of temperature on mycelial growth and sporulation of different pathotype clusters by analyzing 5 isolates from each of the 4 ma-jor clusters. Our findings indicated that the temperature range of 24–32℃ was optimal for the growth and reproduction of rice blast fungus in Taiwan. Among these temperatures, 28℃ was the most favorable. Conditions where the temperature fell below 20℃ or rose above 36℃ significantly restricted the growth and reproduction of the rice blast fungus. All isolates survived, albeit almost completely stopped growing at 36℃. Overall, clusters L4 and L5 exhibited better growth and reproduction at 32℃ com-pared to 24℃. Conversely, L1 and L2 showed superior growth and reproduction at 24℃ rather than 32℃. L4 had a significantly higher reproductive capacity than the other 3 clusters at 24–32℃. Because of this outcome and the consistent rise in average temperature since 2019, it was believed that tem-perature was the main cause of L4 and L5 becoming the prevalent clusters in Taiwan. This study clearly illustrated the impact of temperature changes on the epidemic spread of rice blast fungus in Taiwan. It also offered crucial guidance for developing disease-resistant plans for potential climate change scenarios.
氣候變遷引發的溫度與降雨變化，可能影響某些對濕度和溫度敏感的植物有害生物及其媒介生態，進而改變病害的發生率及其時間和空間分布。先前研究顯示，臺灣稻熱病菌 (Pyricularia oryzae) 可根據對麗江新團黑谷單基因系 (Lijiangxintuanheigu monogenic lines; LTH MLs) 的致病力，分為 5 個病原型菌系，且這些菌系在棲地溫度上存在差異。本研究針對 4 個主要病原型菌系，每個菌系選取 5 個分離株，分析溫度對其菌絲生長與孢子形成的影響。結果顯示，24–32℃為適合臺灣稻熱病菌生長與繁殖的溫度範圍，其中 28℃為最適溫度。當溫度低於20℃或高於 36℃時，稻熱病菌的生長和繁殖受到顯著抑制。所有分離株在36℃下均能存活，但幾乎完全停止生長。整體而言，L4 和 L5 菌系在 32℃下的生長和繁殖優於 24℃；然 L1 和 L2 菌系相對在24℃下表現更佳。特別是 L4 菌系在 24–32℃間的繁殖能力顯著高於其他 3 個菌系。根據 4 個主要病原型菌系在不同溫度下的菌絲生長與孢子形成能力，推測自 2019 年以來平均氣溫持續上升，可能導致 L4 和 L5 成為臺灣近年流行菌系的主要原因。本研究顯示溫度變化對臺灣稻熱病菌流行的影響，並為應對氣候變遷提供重要的抗病策略參考。
Subjects
Climate change
Mycelial growth rate
Pyricularia oryzae
Sporulation
Temperature
氣候變遷
菌絲生長速度
稻溫病菌
產孢
溫度
Publisher
農業部農業試驗所
Type
journal article
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