Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes
Journal
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
Journal Volume
9
Start Page
1520875
ISSN
2571-581X
Date Issued
2025-02-19
Author(s)
Seng-Kai Vong
Chiao-Ming Chen
Arpita Das
Lie-Fen Shyur
Yu-Ming Ju
Huei-Mei Hsieh
Shu-Hui Yang
鳳山熱帶園藝試驗分所
Viola Varga
Sing-Chung Li
Abstract
Pholiota nameko (P. nameko) was identified as an edible mushroom known for its abundance of bioactive compounds. Previous studies suggested that extracts from this mushroom possess antioxidant properties attributed to the presence of phenolic acids, such as 4-hydroxybenzoic acid (4-HBA) and cinnamic acid (CA). These compounds show promise in potentially ameliorating insulin resistance. In this study, the highest levels of 4-HBA and CA were selected from six P. nameko extracts using ultra-high-pressure liquid chromatography (UHPLC) to investigate whether they could improve the glucose uptake resistance induced by palmitate in C2C12 myotubes. Additionally, the protein expression of GLUT4 was examined. The results indicated that P. nameko hot water extract (PN-HW) exhibited the highest total polyphenol content (9.34 +/- 0.31 mg GAE/g) and demonstrates strong free radical scavenging ability (77.4 +/- 1.1%). Furthermore, PN-HW contains notable amounts of 4-HBA (4.02 +/- 0.17 mg/g DW) and CA (2.01 +/- 0.09 mg/g DW). Importantly, the cell viability reveals that PN-HW extracts did not have a toxic effect on C2C12 myotubes. PN-HW significantly enhances glucose uptake at doses of 25 mu g/mL. Hence, it was suggested that PN-HW might contain elevated levels of phenolic acids, potentially mitigating impaired glucose uptake caused by high-fat content and consequently enhancing the glucose uptake capacity of C2C12 cells.
Subjects
Pholiota nameko water extract
insulin resistance
phenolic acids
cinnamic acid
4-hydroxybenzoic acid
SDGs
Publisher
Frontiers Media SA.
Type
journal article