Multiplex polymerase chain reaction assay for pest identification of rice green leafhoppers (Nephotettix, Hemiptera: Cicadellidae)

Lee, Yu-Ju; Huang, Shou-Horng; Dong, Yaw-Jen; Shih, Hsien-Tzung; Hou, Ping-Fu; Sung, I-Hsin

doi:10.1093/jee/toag135

Multiplex polymerase chain reaction assay for pest identification of rice green leafhoppers (Nephotettix, Hemiptera: Cicadellidae)

Journal

Journal of economic entomology

Journal Volume

119

Journal Issue

4

Start Page

2934

End Page

2944

ISSN

1938-291X

Date Issued

2026-08-01

Author(s)

Lee, Yu-Ju

Huang, Shou-Horng

嘉義農業試驗分所

Dong, Yaw-Jen

農業部農業試驗所

Shih, Hsien-Tzung

農業部農業試驗所

Hou, Ping-Fu

Sung, I-Hsin

DOI

10.1093/jee/toag135

URI

https://scholars.tari.gov.tw/handle/123456789/25116

Abstract

Species-specific primers were designed from reference sequences of Nephotettix cincticeps (Uhler), Nephotettix malayanus Ishihara et Kawase, Nephotettix nigropictus (Stål), and Nephotettix virescens (Distant) (Hemiptera: Cicadellidae) and applied in multiplex polymerase chain reaction (PCR) for the molecular identification. The assay yielded distinct amplicons of 263 bp for N. malayanus, 320 bp for N. virescens, 401 bp for N. cincticeps, and 502 bp for N. nigropictus, enabling clear resolution by agarose gel electrophoresis. This method provides a reliable and efficient approach for field surveys and population monitoring of rice green leafhoppers, and is expected to greatly reduce misidentification while providing robust support for pest management decision-making.

Subjects

Nephotettix

COI gene

rice pest

species identification

Publisher

Oxford

Type

journal article