當歸的生產及品質提升之研究
Other Title
Studies on the Production and the Improvement in Quality of Angelica Acutiloba Kitagawa
Start Page
169
End Page
178
ISBN
957-02-4205-1
Date Issued
1999-03
Date
1999-03
Author(s)
賴宏亮
Horng-Liang Lay
Abstract
The study was mainly divided into four diisions to research Angelica acutioba Kitagawa. Firstly, the investigation focused on the relationship of the cultural conditions to the character of plant growth, yield and quality. A series of physiological and ecological studies from seed production, raising of seeding, manuring practice and environment condition etc. to establish optimum cultivation. Secondary, to establish control the bolting method. When bolting occurred in A. acutioba, both plant growth and the physiological activity declined, and once the cambium disappeared and lignification began, the thickening of the root practically came to a halt. At the harvest time, as the plant body was dying, not only was there a marked reduction in production, but also there was a significant decrease in the quality of the crude drug produced. The study author has developed the root cutting technique, a new method which, when applied, results in highly percent control over bolting development. Additionally, the results of this root cutting treatment indicate that the plants can be produced with good crude drug quality at a good yield. Thirdly, to evaluation of quality by physical chemistry. Separated and purified ligustilide, a marker component from A. acutiloba seed, and to establish high-performance liquid chromatographic separation and quantitative determination of ligustilide in the Angelica plant using fluoremetric detection. Another, thin layer chromatographic and high-performance liquid chromatographic methods were applied to the separation of sterols (? -sitosterol, stigmasterol, choles terol, campesterol) and coumarins (bergapten, umbelliferone, scopoletin, xanthotoxin, isopimpinellin, imperatorin) in several parts of different Angelica plants.
Finally, to develop on the mass propagation of A. acutioba through tissue culture. Culture samples with seeds, leaf, petiole, immature and mature, and cultured on the different media supplemented with various combinations of hormones, the results showed that complete plants were regenerated from the somatic embryos, and grew in the fields without any phenotypic abnormalities.
當歸是撒花序科多年生草本植物，使用部位為植物的乾燥根，在神農本草經收載列為中品”主要的功能為補血、行血、潤腸、調經，其適應證為血虛頭痛、心腹諸痛、大便燥結、婦女經閉、經來腹痛、崩漏”自古以來在傳統中醫藥方劑中大量的被使用，是重要的藥材之一“
本研究共分成四個架構對當歸進行探討，其一為栽培條件與生長、生產及品質之間的關係，從採種、育苗、肥培管理到環境條件等，進行一系列的生理、生態之研究，探討最適當的栽培方式”其二為抽苔防止法之建立，當歸具有容易抽苔、開花之特性，抽苔後的植物體會引起生長、生理活性的低下，甚至隨著木質化的進行根部會停止肥大，導致植株枯萎死亡造成收穫上的損失，是栽培上的一大障礙。本研究中建立了切根法，不僅可提高抽苔防止效果並可促進根部的肥大，提高產量。其三為理化學的品質評價，從當歸的種子中單離純化出ligustilide，利用螢光檢測器建立高效液相層析儀（HPLC）的定量分析方法。並利用薄層層析儀（TLC）及HPLC探討? -sitosterol、stigmasterol、cholesterol及campesterol等sterol類的成分與bergapten、umbelliferone、scopoletin、xanthotoxin、isopimpinellin及imperatorin等coumarin類的成分在各種不同當歸及不同部位之組成及分佈。最後為利用組織培養方法達到種苗大量增殖，以未熟種子、葉、葉柄為材料，利用各種培養基及荷爾蒙的組合，探討誘導直接或間接不定胚（somatic embryogenesis）的最適條件，並成功的誘導出植物體，經馴化後可達大量增殖之目的。
Event(s)
1999藥用植物之開發與利用研討會
Proceedings of the Symposium on Development and Utilization of Medicinal Plants 1999
Publisher
農業試驗所
Type
book part
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