茶樹酯酶同功酶與親緣相關之研究

Abstract

以青心烏龍、青心大冇、漢口及其雜交Fl後代，以及再與青心烏龍為父本之雜交F1後代，阿薩姆共15個品種系為材料，分析其酯酶同功嗨之電泳圖譜，藉以比較各種系間相似度。結果顯示電泳圖譜條帶分布有16條，各種系譜條帶分布有9至13條不等，基本酶譜帶有8條，Rf分別為0.12、0.38、0.40、0.47、0.50、0.52、0.59、0.63，出現頻度86.7％以上，而Rf 0.36、0.440.56出現頻度在73.3至53.3％之間，特有條帶Rf 0.17、0.33、0.42、0.45、0.48出現頻度則在40％以下。 經聚類分析其相似度在40 50％時，可分為兩大類群，以青心烏龍為父本之凡代選育新品系及台茶12號為一類群(I)，而青心烏龍、青心大冇、漢口及其雜交F1後代為另一類群(II)，並顯示以青心烏龍為雜交父本之F2代，其與青心烏龍、青心大有、漢口的相似度在30％以下。 The gel electrophoresis was used to analyse the esterase isozymes of the Fl and F2 hybrids of Chin-shin Oolong , Chin-shin Dapang , Hann-koou . There was fifteen species and strains tested. Results showed that total of sixteen bands was detected among them. The different species and strains had eight to thirteen bands. They included eight fundamental bands of Rf = 0.12 , 0.38 , 0.40 , 0.47 0.50 , 0.52 , 0.59 , 0.63. The frequency of reproducible bands was above 86.7%. The bands of Rf z 0.36 , 0.44 , 0.56 was 73.3%-53.3%. The bands of Rf = 0.17 0.33 , 0.42 , 0.45 , 0.48 was behind 40%. The Neis genetic distances of tested species and strains were calculated from Rf values on esterase isozymes. Cluster analysis of this matrix produced a dendrogram which could be separated them into two groups. When the similarity was 40 50 %. The F2 of Chin-shin Oolong and TTES 12 was one group. The Fl of Chin- shin Oolong , Chin-shin Dapang , Hann-koou and parents was another group. The similarity between groups was behind 30%.