Survey of Grapovine Virus Disease in Taiwan
Hueylin Chen Tzeng
|Keywords:||葡萄產業研究與發展研討會專集;Proceedings Of A Symposium On Grape Industry Research And Development||Issue Date:||Dec-1988||Publisher:||台中：農業試驗所||Start page/Pages:||124-130||Source:||葡萄產業研究與發展研討會專集:||Abstract:||
Occurrence of various grapevine virus diseases in Taiwan was investigated. The viral infections of examined samples were determined by mechanical inoculation on various herbaceous indexing hosts, ELISA, electron microscopy, grafting field indexing using specific grapevine virus indicator plants and analyses of nucleic acid and protein contents of the examined grapevine tissue extracts. During the past three years (1985-1988), a total of 810 grapevine samples were collected from different locations. Up to now, leafroll was the only viral disease detected. About 60% of the tested samples collected from the commercial vineyards showed indication of leaf-roll virus infection. Incidence of this infection detected from field grown table grapes like Kyoho, Italia, or wine grapes like Black Queen were over 80%; While rootstocks like 8B, SO4, 1202 and Harmony and other commercial cultivars like Himrod Seedless, Delaware, Buffalo, Neo Muscate, Royal Red, Super Hamburg and Champion were badly affected. The grapevine cultivars currently grown in the Cultivar Collection Centers of Taiwan Tobacco Research Institute, Taiwan Agriculture Research Institute and The Grape Research Center of National Chung Hsing University were also not exempted from the viral infection. Disease incidence detected from these localities were approximately 15-20%; and among them cultivars introduced early from vicinity Asian countries and cultivars introduced from South Africa were the ones affected most. In addition to disease survey, influence of leafroll infection on growth and yield were also examined during the course of study. The impact of leaf roll infection on grapevine production appeared to be most prominent for the two-crop system in which very low or even zero yield due to the viral infection was frequently observed. By electron microscopic study, at least two types of closterovirus-like particles have been discovered from the virus affected samples. Efforts to further characterize the involved viral entities are currently underway.
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