|Title:||Anther culture of papaya (Carica papaya L.)||Authors:||H.S. Tsay
|Keywords:||Sucrose;Light Intensity;Growth Regulator;Chromosome Number;Callus Induction||Issue Date:||Feb-1985||Publisher:||Springer Nature||Journal Volume:||4||Journal Issue:||1||Start page/Pages:||28-30||Source:||Plant Cell Reports||Abstract:||
Papaya (Carica papaya L.) anther containing microspores in tetrad to early-binucleate stages were successfully cultured on 1/2 strength MS salts and vitamins with full strength Na-Fe-EDTA supplemented with 2 mg/l NAA, 1 mg/l BA and 6% sucrose for callus initiation and formation. Highest frequencies of callus induction were obtained when anthers at the uninucleate stage were cultured in the dark. Haploid plantlets and pollen-derived embryoids were obtained from anthers cultured at the uninucleate stage on solidified MS medium containing 3% sucrose without any growth regulators under a low light intensity (1,500 lux). Large quantities of embryoids were obtained when the original embryoids were transferred to MS medium with 3% sucrose and no growth regulators. Cytology of root tips of embryoid-derived plants confirmed the haploid chromosome number of 9 indicating that the embryoids originated from pollen.
|Appears in Collections:||SCI期刊|
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