Anther culture of papaya (Carica papaya L.)

Journal
Plant Cell Reports
Journal Volume
4
Journal Issue
1
Pages
28-30
Date Issued
1985-02
Date
1985-02
Author(s)
H.S. Tsay
C.Y. Su
DOI
URI
Abstract
Papaya (Carica papaya L.) anther containing microspores in tetrad to early-binucleate stages were successfully cultured on 1/2 strength MS salts and vitamins with full strength Na-Fe-EDTA supplemented with 2 mg/l NAA, 1 mg/l BA and 6% sucrose for callus initiation and formation. Highest frequencies of callus induction were obtained when anthers at the uninucleate stage were cultured in the dark. Haploid plantlets and pollen-derived embryoids were obtained from anthers cultured at the uninucleate stage on solidified MS medium containing 3% sucrose without any growth regulators under a low light intensity (1,500 lux). Large quantities of embryoids were obtained when the original embryoids were transferred to MS medium with 3% sucrose and no growth regulators. Cytology of root tips of embryoid-derived plants confirmed the haploid chromosome number of 9 indicating that the embryoids originated from pollen.
Subjects
Sucrose
Light Intensity
Growth Regulator
Chromosome Number
Callus Induction
Publisher
Springer Nature
Type
journal article
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