Phylogenetic diversity and sensitivity to MBI and QoI fungicides of Magnaporthe oryzae in Taiwan
Journal
Journal of Pesticide Science
Journal Volume
38
Journal Issue
4
Pages
194-199
Date Issued
2013-12
Date
2013-12
Author(s)
Abstract
Magnaporthe oryzae (Anamorph: Pyricularia oryzae), the causal agent of rice blast disease, is the major pathogen that reduces the yield of rice worldwide. Melanin biosynthesis inhibitors (MBIs) and strobilurins (QoIs) are common fungicides used to control the disease. One hundred and three M. oryzae isolates from different rice-production areas in Taiwan were evaluated for their molecular phylogeny and sensitivity to MBI and QoI fungicides. Molecular phylogenetic analysis showed that all M. oryzae isolates could be classified into nine genetic groups (A–I) based on the DNA polymorphism amplified by CNS1/MP primers. Predominant ones are groups A (72 isolates) and B (15 isolates). Fungicide tests showed that M. oryzae isolates were sensitive to MBIs (carpropamid and tricyclazole) and less sensitive to QoIs (kresoxim-methyl and azoxystrobin). Amplifying the cytochrome b (cyt b) gene revealed that isolates with low sensitivity to QoIs did not correspond with mutation at codon 129 or 143 in the cyt b gene.
Subjects
Molecular phylogeny
MBIs
QoIs
cytochrome b (cyt b) gene
Publisher
Pesticide Science Society of Japan
Type
journal article
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