由茉莉花(Jasminum sambac)分離之一種新potyvirus之特性研究
Other Title
Characterization of a New Potyvirus Isolated from Jasmin (Jasminum sambac (L.)Ait) in Taiwan
Journal
植物病理學會刊
Journal Volume
13
Journal Issue
1
Pages
69-84
Date Issued
2004-03
Date
2004-03
Author(s)
林宜螢
陳滄海
張清安
Lin, Y. Y.
Chen, T. H.
Chang, C. A .
Abstract
A previously undescribed virus disease that caused severe yellow mosaic on leaves of jasmin plants was found in 1995 at Kaohsiung and Pingtung, Taiwan. The first discernible symptom observed is irregular yellow spot. Later, the spot advanced and making the whole leaf yellowing. Three major symptom types, i.e. symptomless virus infection, irregular yellow spot and severe yellow mosaic were evidenced in the field. The virus disease is a complex disease, more than three filamentous viruses were observed in crude saps of field jasmin. A flexuous filamentous virus of 720-750 nm in particle size, was successfully isolated by mechanical inoculation from diseased jasmin showing yellow mosaic symptom. It is the first report of jasmin virus disease in Taiwan. The thermal inactivation point of the virus was 50-55 °C, dilution end point was 10-3 to 10-4 and the longevity in vitro was 2-3 days at 24°C and more than 10 months at -70 °C. Pinwheel-shaped and laminated inclusion bodies typical of potyvirus infection were found in local lesion of Chenopodium amaranticolor. Cellular organelles alterations of nuclei, chloroplast, mitochondria in virusinfected leaf of jasmin showing yellow mosaic symptom were also observed by electron microscopy. It had a very narrow host range restricted to three species in chenopodiaceous plants among 56 tested plant species belonging to 12 families. Attempts to transmit the virus by aphids using C. quinoa and C. amaranticolor as source plants failed. Protein with a molecular weight of 32 kDa was detected where purified virus was denatured with sodium dodecyl sulfate (SDS) and analyzed by electrophoresis in polyacrylamide gels (SDS-PAGE) and by western blotting. An antiserum with a titre of 512 was obtained by immunizing a white rabbit with the purified virions. In Western blotting, indirect ELISA, SDS-agar gel double diffusion test, the antiserum against the jasmin virus isolate reacted strongly with its homologous antigens, but not to antigens of fourteen other potyviruses. In order to further characterize the virus at the molecular level, CP genes of jasmin virus isolate was cloned, sequenced and analyzed. A sequence of 1289 nucleotides (nts) was found and the percent of nucleotide identities of the CP gene and the NCR were less than 69% and 54%, comparing to twenty potyviruses, respectively. Similarly, percent identities of CP amino acid sequence to those of twenty other known potyviruses were all below 69%. The virus was identified as a new potyvirus species on the basis of its particle morphology and size, physical properties, cytopatholoy, serological characteristics and percent of nucleotide and amino acid sequences identity, and a name, Jasmin virus T, JaVT, was previsionally proposed. It is the first report about successful virus isolation from jasmin in the world.
Subjects
茉莉花
病毒鑑定
茉莉病毒T
Jasmin
Potyvirus
Virus identification
Jasmin virus T
Publisher
中華民國植物病理學會
Type
journal article
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