Molecular Characterization and Phylogenetic Analysis of Turnip mosaic virus Collected from Calla Lily
Other Title
分離自彩色海芋之蕪菁嵌紋病毒之分子特性及其類緣演化分析
Journal
植物病理學會刊
Journal Volume
15
Journal Issue
1
Pages
1-8
Date Issued
2006-03
Date
2006-03
Author(s)
Abstract
The full-length genomic sequences of two isolates of Turnip mosaic virus (TuMV) from calla lily (Zantedeschia spp., Araceae) , RC4 and YC5, were determined from 5'-RACE and overlapped viral cDNA clones derived from reverse-transcription polymerase chain reaction (RT-PCR) using TuMVspecific primers. Both TuMV-RC4 and TuMV-YC5 showed similar genomic features. When compared with TuMV isolates available in GenBank, sequences coding for P1, P3 and 6K2 proteins were identified as the most varible regions. Based on the variablilty of amino acid sequences at the amino acids positions of 471-490 in CIP region, TuMV isolates were divided into two groups. Among 42 TuMV isolates compared, only Q-Ca (Acc. No. D10927) and C1-isolate (Acc. No. AF394601) have a sequence of GSQPVxMxDxVxMxKIGVTL, while RC4, YC5, and other 38 isolates with a sequence of WLTAEYARLGANVEDRRDV. The protease recognition tetrapeptides for P1 protease and nuclear inclusion a protease (NIa-Pro) are variable among TuMV isolates, but both of RC4 and YC5 are identical. The phylogenetic analyses based on the comparison of the deduced amino acid sequences of the complete polyproteins with those of other 30 TuMV isolates, and the coat protein regions with those of other 59 TuMV isolates revealed that RC4 and YC5 are both classified in the group that contains isolates belonging to the Brassica pathotype.
蕪菁嵌紋病毒(Turnip mosaic virus TuMV)屬於馬鈴薯Y屬病毒(Potyvirus)，其寄主範圍相當廣泛。本研究針對兩種分離自彩色海芋(Zantedeschia spp.)的TuMV病毒分離株RC4及YCS，利用5’-RACE及TuMv之專一性引子進行反轉錄-聚合釀鏈鎖反應，由相互革疊的cDNA片段選殖解序後，各別罕得基因體RNA均含9832釀個核苜酸，此二病毒分離株之全長度基因體核酸序列並已登錄於基因庫(GenBank)，序號分別為為AY134473及AF530055。RC4及YC5之基因體架構相同，可對應轉譯出一含3164個氨基酸之大蛋白。5’端之序列包含有四個腺嘌呤核苷酸(adenylated nucteotides)，大蛋白之第一個胺基酸轉譯之起始位(AUG)在5’端起第129-131 nts。與登錄於GenBank之TuMV戲不分離株比對結果，發現P1、P3及6k2等蛋白在TuMV分離株間變異性最大。對應CIP蛋白之第471-490胺基酸出現差異性，在所比對的42個分離株中，僅有Q-Ca (Acc. No. D10927)及C1 (Acc. No. AF394601)分離株呈現GSQPVxMxDxVxMxKIGVTL序列，而RC4、YC5與其他38個分離株則均為WLTAEYARLGANVEDRRDV序列型態。RC4及YC5乃分離自天南星科之海芋分離株，但二者均能感染十字花科且造成植株系統性嵌紋病徵。進一步根據與其他30個具有全長度大蛋白
胺基酸序列之TuMV分離株，以及與其他59個TuMV分離株之鞘蛋白胺基酸序列所得之遺傳演化分析結果顯示，RC4與YC5乃同列於大部分由分離自十字花科白菜屬(Brassica spp.)之TuMV所組成的群組，然而RC4與YCS之間仍有一段遺傳距離。此種遺傳距離的差異性是否因RC4及YC5乃分離自不同的海芋品系及地理源頭所影響，值得進一步探討。
Subjects
Turnip mosaic virus
complete genomic sequence
phylogenetic analysis
蕪菁嵌紋病毒
全長度核酸序列
演化分析
Publisher
中華民國植物病理學會
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
15-1-1-8.pdf
Size
162.15 KB
Format
Adobe PDF
Checksum
(MD5):eb1ef6f72052ed06b70461741366ebbd