|Title:||杏鮑菇褐斑病菌之半選擇性培養基||Other Titles:||A Semiselective Medium for Detecting Gliocladium roseum, the Causal Agent of King Oyster Mushroom Brown Spot||Authors:||陳錦桐
|Keywords:||杏鮑菇;褐斑病菌;麥芽糖一亞硝酸鈉半選擇性培養基;Pleurotus eryngii;Gliocladium roseum;maltose-NaNO2 (MNa) semiselective medium||Issue Date:||Jun-2004||Publisher:||中華民國植物病理學會||Journal Volume:||13||Journal Issue:||2||Start page/Pages:||107-116||Source:||植物病理學會刊||Abstract:||
評估十二種不同的碳素源與十二種氮素源對杏鮑菇褐斑病菌(Gliocladium roseum Bainier)菌絲生長的影響，結果發現麥芽糖、可才容吐澱粉、蔗糖及己六醇等碳素源和硝酸鹽與亞硝酸噸之氮素源，均有助於本菌菌絲的生長，其中以麥芽糖搭酊亞硝酸鈉效果最好。利用含有麥芽糖和亞硝酸鈉的蔡氏修正培養基，評估不同化學藥劑對本菌生長的影響，證實腐絕5 ppm、福多寧400 ppm、鏈黴素500 ppm，青黴素500 ppm及氯黴素500 ppm等不具有抑制本菌菌絲生長之功效。將麥芽糖30公克、亞硝酸鈉2公克、磷酸氫二鉀1公克、硫酸鎂0.5公克、氯化鉀0.5公克、硫酸鐵0.01公克、洋菜粉15公克及蒸餾水1公升均勻混合，高溫高壓滅菌後，逐一添加福多寧200 ppm、腐絕4 ppm、鏈黴素200 ppm、青黴素200 ppm及氯黴素200 ppm等藥劑，製成(MNa Maltose-NaNO2)半選擇性培養基，可以有效偵測木屑中仔活的杏鮑菇褐斑病菌。利用MNa半選擇性培養基，檢測本菌的感染源，結果在山黃麻木屑與菇舍栽培場鄰近的樹林土壤中，發現有本病原菌的仔在，尤其在菇舍空氣濾網上，有人量的褐斑病菌附著污染。
Twelve carbohydrates and twelve nitrogenous compounds were evaluated for their effects on mycelial growth of Gliocladium roseum isolates GR-12 and GR-28, the causal agent of brown spot of king oyster mushroom Pleurotus eryngii . Among those carbon-source compounds, sucrose, sorbitol, starch and maltose were more effective than other carbohydrates to enhance the growth of G. roseum. For nitrogenous compounds, NaNO3, NaNO2 and KNO3 were more effective to enhance growth of the pathogen. Seven pesticides were separately added into the basal medium (a modified Czapek's medium containing 3% (w/v) maltose and 0.2% (w/v) NaNO2 , MNa medium) to investigate their suppressive effectiveness for mycelial growth. Data showed that mycelial growth of the pathogen was not inhibited by flutolanil at 200 ppm, mertect at 4 ppm, streptomycin sulfate at 500 ppm, chloramphenicol at 500 ppm and penicillin G at 500 ppm. Therefore, maltose-NaNO2 semiselective medium (MNa semiselective medium) consisting of 30 g maltose, 2 g NaNO2, 1 g K2HPO4, 0.5 g MgSO4 7H2O, 0.5 g KCl, 0.01 g FeSO4 7H2O, 20 g agar, 200 ppm flutolanil, 4 ppm mertect, 200 ppm streptomycin sulfate, 200 ppm chloramphenicol, 200 ppm penicillin G and 1L distilled water was formulated for the enumeration and isolation of G. roseum from infested sawdust. The results showed that G. roseum could be accurately detected from artificially and naturally infested sawdust by use of MNa semiselective medium. Population density of the pathogen in naturally infested sawdust and soil was 0-1.0 102cfu/g sawdust and 0-3.0 102cfu/g soil, respectively. High population density of the fungus was also detected from air filter of the mushroom cultivation room by MNa semiselective medium.
|Appears in Collections:||植物病理組|
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