Pea Seed-borne Mosaic Virus Causing Pea Mottling in Taiwan

Chin-An Chang; Cheng-Min Chen; Hui-Chi Chen; Ting-Chin Deng; C.H. Huang; 張清安; 陳建名; 陳慧琦; 鄧汀欽; 黃秋雄; Chin-An Chang;Cheng-Min Chen;Hui-Chi Chen;Ting-Chin Deng;C.H. Huang; 張清安;陳建名;陳慧琦;鄧汀欽;黃秋雄

Pea Seed-borne Mosaic Virus Causing Pea Mottling in Taiwan

Other Title

引起豌豆葉部斑紋病徵之豌豆種媒嵌紋病毒之鑑定

Journal

植物保護學會會刊

Journal Volume

31

Journal Issue

4

Pages

366-376

ISBN

0577-750X

Date Issued

1989-10

Date

1989-10

Author(s)

Chin-An Chang

Cheng-Min Chen

Hui-Chi Chen

Ting-Chin Deng

C.H. Huang

張清安

陳建名

陳慧琦

鄧汀欽

黃秋雄

URI

https://scholars.tari.gov.tw/handle/123456789/4688

https://tpl.ncl.edu.tw/NclService/JournalContentDetail?SysId=A89021477

Abstract

A virus isolate (PS8) causing mottling or mild mosaic foliar symptoms on pea was collected from a field in Ear-Lin area in 1987. The virus was successfully transmitted to pea cultivar “Hsin-Jan” by manual inoculation and by aphids with non-persistent manner. Cytoplasmic cylindrical (Cl) and amorphous (Al) inclusions were observed in infected cells by light microscopy. Flexuous rod-shaped paiticles with mean length of 734 nm were detected in infected leaf extracts. Among twenty four plant species inoculated only pea and broad bean were systemically infected, and the host reactions were similar to those of described pea seed-borne mosaic virus (PSbMV) isolates from the USA. The virus was successfully purified by differential and Cs2 SO4 equilibrium centrifugation. The purified capsid and Cl proteins consisted of single species of protein monomer with molecular weights of 29 and 74 kilo-daltons, respectively. In reciprocal SDS-immunodiffusion tests, PS8 was found serologically indistinguishable from a PSbMV isolate from the USA (provided by G. I. Mink, Washington State Univ, U.S.A.), related to, but distinct from, black eye cow pea mosaic virus, passionfruit woodiness virus, and potato virus Y, and unrelated to eleven other potyviruses tested. On the basis of these results, PS8 was recognized as an isolate of PSbMV. This is the first record for the occurrence of PSbMV in Taiwan. 民國76年由二林地區所栽植之新珍甜豌豆分離獲得一個病毒分離株(PS8)，可於新珍豌豆葉部造成輕微斑紋至嵌紋病徵。在實驗室中PS8可經由人工接種及桃蚜媒介至健全豌豆，並造成相同病徵，於光學顯微鏡下罹病株葉片下表皮細胞內可觀察到PSS所形成之圓柱狀內含體(CI)及不定形內含體(AI)。於電子顯微鏡下可於罹病植株汁液中發現平均長度約734nm之長絲狀病毒顆粒。接種5科24種植物發現，PS8只能感染豌豆及蠶豆。此種窄狹之寄主範圍特性與國外報告之豌豆種媒嵌紋病毒極其類似。經高低速交替及密度階層平衡離心，吾人已將PS8由豌豆中純化成功，其鞘蛋白及圓柱狀內含體蛋白之分子量分別經電泳分析估計為29K及74K。在血清學鑑定方面，供試之14種抗血清中，唯有由美國華盛頓州立大學G. I. Mink所提供之PSbMV抗血清可與PS8抗原反應，而且其反應沈澱帶與PS8同源沈澱帶癒合，顯示二者間血清性質極為接近。再加上PSbMV抗血清與供試13種不同PVY群病毒抗原之反應關係與PS8抗血清所獲結果完全相同，因此進一步證實PSbMV與PS8間血清性質應完全相同，PS8與黑眼豇豆嵌紋病毒(BICMV)，馬鈴薯Y病毒(PVY)及百香果木質化病毒(PWV)具血清類綠關係，但與其他11種供試病毒不具血緣關係。由各項特性鑑定PS8應屬豌豆種媒嵌紋病毒之一分離株。此乃PSbMV發生於本省之首次記錄。

Subjects

pea seed-borne mosaic virus

PSbMV

serology

identification

amorphous inclusion

碗豆種媒嵌紋病毒

血清學

鑑定

Publisher

植病保護學會

Type

journal article

File(s)

Name

31-4-366-376.pdf

Size

1.7 MB

Format

Adobe PDF

Checksum

(MD5):78476e609a5db8a502c8a3b72a1f5c20