|Title:||臺灣玉米矮化嵌紋病毒B型系統與甘蔗嵌紋病毒系統之病原性、血清學及其發生之研究||Other Titles:||Comparative Studies on Pathogenlcity, Serology, and Occurrence of Maize Dwarf Mosaic Virus B Strain and Sugarcane Meaalc Virus Strains in Taiwan||Authors:||鄧汀欽
|Keywords:||玉米矮化嵌紋病毒;甘蔗嵌紋病毒;抗血清;病原性;流行性;Maize dwarf mosaic virus;Sugarcane mosaic virus;Antiserum;Pathogenicity;Prevalence||Issue Date:||Apr-1987||Publisher:||植病保護學會||Journal Volume:||29||Journal Issue:||2||Start page/Pages:||123-133||Source:||植物保護學會會刊||Abstract:||
根據病毒的血清學特性及對六個玉米栽培品種的病原性，玉米矮化嵌紋病毒B型系統(MDMV-B)可與各個甘蔗嵌紋病毒(SCMV)系統區別。一般MDMV-B比各個SCMV系統對供試的玉米栽培品種都有較強的病原性，MDMV-B可在任何季節感染臺農351，且使各品種玉米發病快而感染率高，其他SCMV系統僅能在高溫期感染臺農351，若在低溫期則於二十四天接種後仍未出現病徵，但MDMV-B接種後七天即開始發病。瓊脂擴散反應中，MDMV-B抗血清對各個SCMV系統均有異源(heterologous)反應，其沈澱帶與同源的(homofogous) MDMV-B所形成的呈刺狀(spur)；另純化S-1 isofate病毒製成抗血清，以MDMV-B感染之玉米粗汁液交互吸收，此抗血清不再與MDMV-B反應，但是與各個SCMV系統均有同等之反應。以雙層抗體法免疫酵素分析(DAS-EUSA)，當各個SCMV系統和MDMV-B抗體反應時，所有SCMV系統的ELISA值均明顯低於同源的MDMV-B，因此得以把MDMV-B從SCMV系統中區分出來；當上述抗原和S-1 isolate的抗血清反應時，所有SCMV系統的讀值則明顯高於MDMV-B；若再把S-1 isolate的抗血清以MDMV-B交互吸收，則該血清完全不與MDMV-B反應，但是與各個SCMV系統均有同等之反應。利用此一系列反應，至田間大量調查，可檢出屬於SCMV的系統，且把MDMV-B從其中鑑別出來。於1983冬季至1986春季，在臺灣各玉米產區篩檢毒素病標本，結果從1叩處來源收集的689個檢體中，鑑定有512個是MDMV-B，33個是SCMV系統，30個是兩者混合，114個是兩者皆負反應者。顯示MDMV-B是目前臺灣玉米嵌紋病最主要之病原。
Maize dwarf mosaic virus B strain (MDMV-B) was distinguished from strains of sugarcane mosaic virus (SCMV) based on their serological properties of virus particles and pathogenicity to six different corn cultivars. In general, MDMV B was more virulent to alt the corn cultivars tested than strains of SCMV. It consisently infected cuitlvar “Tainong 351”, while strains of SCMV could only infect this cultivar during summer season. In winter, no symptom was found on this cultivar even 24 days after inoculation with SCMV strains, while symptoms of MDMV-B were observed 7 days after inocti1atn. Antiserum to MDMV-B reacted to strains of SCMV Indistinguishably bet the homologous precipitation line consistently spurred over the heterologous precipitation lines in SDS-immunodiffusicm tests. In a reciprocal test, antiserum to S-1. isolate of SCMV gave intinguishable reactions to all strains of SCMV but their precipitation lines also spurred over that of MDMV-B. When antiserum to SCMV S-1 was cross-absorbed with a crude sap of MDMV-l3 infected corn, it no longer reacted with MDMV-B but still reacted conspicuously with strains of SCMV. Using DAS-ELISA, strains of SCMV could also be distinguithed from MDMV-B by the apparent lower ELISA values than homologous reactions when antiserum to MDMV-B was used. Reciprocally, antiserum to S-1 isolate of SCMV consistently gave higher readings to strains SCMV than those to MDMV-B When this antiseum was cross-absorbed with MDMV-B, it reacted only with strains of SCMV but not with MDMV-B. Thus, the method was used to differentiate MDMV-B from SCMV strains in a large-scale tick survey from 1983 to 1986. In a total of 689 samples collected from 100 corn fields, 512 samples were singly infected with MDMV-B, 33 samples were singly infected with SCMV, and 30 samples were mixed infected with MDMV-B and SCMV, whereas
114 samples were negative to both antisera. These results indicated that MDMV-B was the major causal virus of corn mosaic diseases in Taiwan.
|Appears in Collections:||植物病理組|
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