https://scholars.tari.gov.tw/handle/123456789/4795
Title: | 進口梨接穗花枯病之鑑定與防治 | Other Titles: | Identification and Control of Pear Blossom Blast in Imported Pear Scions | Authors: | 蔡佳欣 安寶貞 鄧文玲 Tsai, C.H. Ann, P.J. Deng, W.L. |
Keywords: | 梨接穗;花枯病;Pseudomonas syringae pv. Syringae;pear scion;pear blossom blast;Pseudomonas syringae pv. syringae | Issue Date: | Sep-2015 | Publisher: | 農業試驗所 | Related Publication(s): | 農業試驗所特刊第184號 | Start page/Pages: | 23-30 | Source: | 台灣新浮現之重要作物病害及其防治研討會專刊 | Conference: | 台灣新浮現之重要作物病害及其防治研討會 Proceedings of the Symposium on Important New Emerging Crop Diseases Taiwan and Their Controls |
Abstract: | 進口梨接穗花枯病(Pear blossom blast),在台灣最早於民國103 年宜蘭三星鄉農友發現所購買的日本梨穗,枝條有異常凹陷黑斑,將皮削開後可見其內部組織,呈現壞疽狀斑點病癥,該農友通報防檢局將樣品後送至本所檢驗後,由枝條黑斑組織可分離出一種細菌,並鑑定該細菌為Pseudomonas syringae pv. syringae,為歐美國家梨樹花枯病的病原細菌。該菌可感染梨樹造成花枯、芽枯及葉斑等病徵。當環境適合病害發生時,可造成顯著的梨果產量損失。在診斷鑑定上可將疑似感染病菌之樣品於King’s B培養分離病原細菌,所得之細菌測試菸草過敏性反應,並以Biolog、16S rDNA序列分析及專一性引子等方式鑑定該菌為Pseudomonas syringae。進一步再將該細菌接種至梨、紫丁香及豆莢測定其病原型。為避免該病菌於國內蔓延造成經濟損失,已篩選出4 種藥劑供緊急防治用。 In 2014, Pear blossom blast in imported pear scions was first found in Sanshing township, Yilan county. The abnormal sunken black spots were observed in the surface of some pear scions imported from Japan. The internal tissues of the diseased scions showed necrotic spot symptom. The bacteria isolated from necrotic tissues of the scions were identified as Pseudomonas syringae pv. syringae. The bacteria can cause blossom blast, bud blight, leaf spot symptoms and so on. When weather favors disease development, the disease could result in reducing yields significantly. In diagnosis, the bacteria isolated from infected scions on King’s B medium could test the hypersensitive reaction in tobacco plant. The bacteria can be further identified as Pseudomonas syringae based on Biolog, 16S rDNA sequence analysis and specific primers. The pathovar syringae of P. syringae can be tested by inoculation of the bacteria on pear, lilac, and bean pod. For avoiding economic loss, 4 emergency agrochemicals were selected for the disease control. |
URI: | https://scholars.tari.gov.tw/handle/123456789/4795 | ISBN: | 978-986-04-5982-1 |
Appears in Collections: | (4)植物病理組 |
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no184-3.pdf | 563.51 kB | Adobe PDF | View/Open |
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