|Title:||Reduction of Rhizoctonia damping-off in Chinese cabbage seedlings by fungal protein||Other Titles:||利用真菌蛋白激活子防治白菜立枯絲核病||Authors:||Tzu-Yang Hsieh
|Keywords:||Alternaria;elicitor;defense;disease resistance;fungal pathogens;鏈格孢菌;激活子;防禦反應;抗病性;真菌性病原菌||Issue Date:||Mar-2016||Publisher:||中華植物保護學會；中華民國植物病理學會；台灣昆蟲學會||Journal Volume:||58||Journal Issue:||1||Start page/Pages:||1-8||Source:||植物醫學||Abstract:||
Total proteins were purified from 12 Alternaria species and tested for their ability to promote growth and disease reduction in Chinese cabbage. Our results indicated that proteins purified from some but not all Alternaria spp. were capable of promoting plant growth, stimulating root elongation and the formation of lateral roots, and reducing damping-off incidence caused by Rhizoctonia solani. Proteins purified from a cherry isolate designated APR01 exhibited most promising results in suppressing damping-off by 30%. APR01 isolate was identified as A. tenuissima based on cultural and morphological characteristics and sequence analysis of rDNA internal transcribed spacer (ITS). In vitro assays revealed no fungistatic activity of APR01 protein against R. solani. APR01 proteins with M.W. greater than 10 kDa displayed ability to reduce R. solani damping-off, whereas proteins M.W. less than 10 kDa had no effects. APR01 proteins heated at temperatures higher than 50°C completely or nearly abolished the ability to reduce disease, indicating that the major components contributing to disease reduction could be enzymes or heat-labile proteins. Nonetheless, our studies have demonstrated the possibility of fungal proteins in the reduction of a soil-borne disease, likely via the induction of plant defense mechanisms.
|Appears in Collections:||植物病理組|
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