Reduction of Rhizoctonia damping-off in Chinese cabbage seedlings by fungal protein
Other Title
利用真菌蛋白激活子防治白菜立枯絲核病
Journal
植物醫學
Journal Volume
58
Journal Issue
1
Pages
1-8
Date Issued
2016-03
Date
2016-03
Author(s)
Abstract
Total proteins were purified from 12 Alternaria species and tested for their ability to promote growth and disease reduction in Chinese cabbage. Our results indicated that proteins purified from some but not all Alternaria spp. were capable of promoting plant growth, stimulating root elongation and the formation of lateral roots, and reducing damping-off incidence caused by Rhizoctonia solani. Proteins purified from a cherry isolate designated APR01 exhibited most promising results in suppressing damping-off by 30%. APR01 isolate was identified as A. tenuissima based on cultural and morphological characteristics and sequence analysis of rDNA internal transcribed spacer (ITS). In vitro assays revealed no fungistatic activity of APR01 protein against R. solani. APR01 proteins with M.W. greater than 10 kDa displayed ability to reduce R. solani damping-off, whereas proteins M.W. less than 10 kDa had no effects. APR01 proteins heated at temperatures higher than 50°C completely or nearly abolished the ability to reduce disease, indicating that the major components contributing to disease reduction could be enzymes or heat-labile proteins. Nonetheless, our studies have demonstrated the possibility of fungal proteins in the reduction of a soil-borne disease, likely via the induction of plant defense mechanisms.
Subjects
Alternaria
elicitor
defense
disease resistance
fungal pathogens
鏈格孢菌
激活子
防禦反應
抗病性
真菌性病原菌
Publisher
中華植物保護學會；中華民國植物病理學會；台灣昆蟲學會
Type
journal article