|Title:||植物萃取物防治十字花科蔬菜炭疽病之篩選模式||Other Titles:||Screening model of plant extracts for control of cruciferous vegetable anthracnose caused by Colletotrichum higginsianum||Authors:||謝廷芳
|Keywords:||中藥草植物;酒精萃取;水萃取;十字花科蔬菜炭疽病;抑菌;誘導抗病;Chinese herbs;ethanol extract;water extract;cruciferous vegetable anthracnose;Colletotrichum higginsianum;pathogen suppression;disease control||Issue Date:||Jun-2016||Publisher:||中華植物保護學會; 中華民國植物病理學會; 台灣昆蟲學會||Journal Volume:||58||Journal Issue:||2||Start page/Pages:||59-68||Source:||植物醫學||Abstract:||
Fifty-seven plant materials including Chinese medicinal herbs and aromatic herbs extracted by ethanol and water were evaluated for their suppression of disease severity and lesion number of cruciferous vegetable anthracnose caused by Colletotrichum higginsianum PA- 01. Each plant extract was diluted into 100-fold extract solution with distilled water and sprayed on the leaf surface of Pak-choi, one kind of cruciferous vegetables, then the spore suspension with 105 spore/ml of anthracnose pathogen PA-01 was inoculated on the extract-treated plants. Total 44 of 114 plant extracts showed an effective disease inhibition. Among 44 plant extracts including 24 ethanol extracts and 20 water extracts, 31 of them with 100-fold dilution showed strong inhibition ability on spore germination of C. higginsianum PA-01, but not for other 12 water extracts. Those water extracts showing an ability of anthracnose disease inhibition but not for spore germination inhibition were presumed to have the ability to induce disease resistance. The plant extracts with strong inhibitory effect of spore germination were further tested for their inhibition concentration. Data showed that the ethanol extracts of 100-fold-diluted ginger, 200-fold-diluted sage, camphor leaf, and Saposhnikoviae Radix, 800-fold-diluted rosemary, areca nut, and Rhei Rhizoma completely inhibited spore germination and appressorial formation of C. higginsianum PA-01. In addition, the ethanol extracts of 200-fold-diluted rosemary, and 400-fold-diluted Rhei Rhizoma, as well as the water extracts of 100-fold-diluted areca nut, and 200-fold-diluted ginger showed the effective suppression of anthracnose disease. In this study, it was proved that the screening model for selection of potential plant extracts with plant inoculation method first, then analyzed the antifungal activity of the extracts with disease inhibitory effect was not missed the plant extracts with an ability to induce disease resistance.
|Appears in Collections:||植物病理組|
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