|Title:||Phytoplasmas of two 16S rDNA groups are associated with pear decline in Taiwan||Other Titles:||造成台灣地區梨樹衰弱病之二群植物菌質體之研究||Authors:||Shu-Ling LIU
|Keywords:||Grafting;Insect transmission;Phytoplasma detection;Transmission electron microscope;Vectors;嫁接;昆蟲咬食傳菌試驗;植物菌質體偵測;穿透式電子顯微鏡;媒介昆蟲||Issue Date:||Jul-2011||Publisher:||中央研究院植物暨微生物學研究所||Journal Volume:||52||Journal Issue:||3||Start page/Pages:||313-320||Source:||Botanical Studies||Abstract:||
Polymerase chain reaction (PCR)-based detection strategies were adopted to examine the etiology and vectorship of pear decline in Taiwan (PDTW). 16S rDNA sequences were amplified from total DNAs prepared from PDTW-affected pear trees Pyrus serotina Rehd. cv. Hengshan and pear psyllid Cacopsylla chinensis using PCR. According to the sequence analyses, C. chinensis carried phytoplasmas of two 16S rDNA groups, PDTW (group 16SrX) and PDTWII (a newly discovered group 16Sr11 phytoplasma associated with pear decline in Taiwan), that were associated with PDTW-infected pear trees. The 16S rDNA sequences of PDTW and PDTWII phytoplasmas that were amplified from diseased pear trees were identical to those from C. chinensis. Transmission trials of phytoplasmas associated with PDTW to healthy pear plants were successfully performed with C. chinensis. One of the 17 tested plants was infected with both PDTW and PDTWII phytoplasmas while ten were infected with PDTWII phytoplasma. In grafting tests, both PDTW and PDTWII phytoplasmas were effectively and separately transmitted from diseased pear to periwinkle plants (Catharanthus roseus). For detection purposes, specific primers were developed and adopted to detect both PDTW and PDTWII phytoplasmas by nested or semi-nested PCR. Transmission electron microscopic examinations revealed phytoplasma bodies in the sieve tubes and phloem parenchyma cells of diseased pears and in the intestinal wall cells of C. chinensis and C. qianli.
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