Colloidal silver-based lateral flow immunoassay for rapid detection of melamine in milk and animal feed
Journal
Materials Chemistry and Physics
Journal Volume
231
Pages
121-130
Date Issued
2019-07
Date
2019-07
Author(s)
Abstract
An immunosilver chromatographic strip based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in milk and animal feed. Colloidal silver nanoparticles (AgNPs), of a diameter of approximately 10.4 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. The anti-MEL monoclonal antibody (mAb) was successfully immobilized on the surface of AgNPs by ionic interactions and characterized using XRD, UV-Vis, TEM and XPS analyses. Surface modification of Ag-mAb conjugates with varying cross-reactivity and thermo-stability was employed to design and prepare labels for use in a lateral flow immunoassay (LFIA) to examine whether these factors affect the performance of the assay. The results showed that no cross-reactions with homologues cyanuric acid, ammeline or ammelide were found, and the test strip remained stable after storage for one year at room temperature. Under optimized conditions, a detection limit of 0.30 ppm for standard melamine was achieved, and limits of detection of 0.750 ppm and 0.875 ppm were obtained in milk and animal feed, respectively.
Subjects
Colloidal silver
Lateral flow immunoassay
Melamine
Test strip
Publisher
Elsevier
Type
journal article
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