以18S rRNA區分基徵草蛉Mallada basalis (Walker) 及安平草蛉 Mallada desjardinsi (Navás) (脈翅目：草蛉科)

Abstract

曾於台灣生物防治上研究過之草蛉有基徵草蛉Mallada basalis (Walker) 及安平草蛉Mallada desjardinsi (Navás)，前者在台灣目前被大量飼養應用於田間防治害蟲。運用分生技術可區分這兩種草蛉，以PCR 放大此兩種草蛉的部分18S rRNA，其序列相似值為 0.97。依據其序列差異處，設計具種專一性引子，可各自增幅放大此二種草蛉之專一性DNA 片段，長度各為 950 及 826 bp，可於電泳圖上清楚分辨。 此具種專一性聚合酶連鎖反應方法，可應用於基徵草蛉室內大量飼養及自田間採集草蛉更新室內品系時，監控、確認草蛉種類。 Mallada basalis (Walker) and Mallada desjardinsi (Navás) have been studied as biological control agents in Taiwan. Of the two green lacewings, Mallada basalis is being mass reared and used in pest control in Taiwan. Molecular techniques were applied to characterize these two green lacewings. Their partial 18S rRNA was amplified by polymerase chain reaction. The pairwise similarity of sequences from the two species was 0.97. PCR with species-specific primers based on 18S rRNA sequences provided partial 18S products of 950 and 826 bp differentiates, allowing these two species to be readily visualized after electrophoresis. Such species-specific PCR could be used to evaluate individuals for contamination during mass rearing of Mallada basalis, or to confirm the identity of field-collected specimens.